The spread of Northern [Exserohilum turcicum (Pass.) K.J. Leonard & Suggs] and Southern [Bipolaris maydis (Y.Nisk. & C. Miyake) Shoemaker] leaf blight diseases in main corn (Zea mays L.) production areas in Turkey The spread of Northern [Exserohilum turcicum (Pass.) K.J. Leonard & Suggs] and Southern [Bipolaris maydis (Y.Nisk. & C. Miyake) Shoemaker] leaf blight diseases in corn was investigated in 5 geographical regions including 13 different provinces between the years of
Northern corn leaf blight caused by Exserohilum turcicum is one of the most significant diseases in maize‐growing fields, resulting in yield reduction worldwide. Genetic diversity and mating‐type of E. turcicum were determined using isolates collected from 7 important maize cultivation provinces in 4 different geographic regions of Turkey during 2014–2015. Multilocus haplotypes of E. turcicum isolates were characterized using 13 simple sequence repeats (SSR) primer pairs, which were amplified in PCR multiplex reactions. The evaluation of mating‐type ratio generated from the E. turcicum population was by χ2 significance test. MAT‐1 and MAT‐2 mating‐type were shown in the population; however, the χ2 significance test did not support the sexual reproduction hypothesis in any region. The E. turcicum population was found to produce predominantly asexual reproduction in Turkey. Numerous E. turcicum isolates showed very high genetic similarity and did not show a distinct cluster dependent on region or mating‐type on a dendrogram of genetic diversity. This result showed that one‐hundred per cent similarity in most of the collected isolates from different regions might also support long‐distance migration.
Bipolaris sorokiniana buğday ve arpada kök çürüklüğü yapan en önemli etmenlerden biridir. Buğday ve arpada tohum, başak, yaprak ve köklerde hastalık oluşturarak bitkilerde önemli ürün kayıplarına yol açmaktadır. Farklı agro-ekolojik bölgelerden toplanan kök ve kök boğazında çürüklük belirtisi gösteren buğday bitkilerinden izole edilen Bipolaris spp. izolatları morfolojik teşhis edilmiştir. Bu çalışmada B. sorokiniana, B. spicifera, ve B. australiensis olarak teşhis edilmiş olan 49 izolat kullanılmıştır. Yapılan patojenite çalışması sonucunda B. sorokiniana izolatlarının ikisi hariç hepsinin patojen olduğu ve hastalık şiddetlerinin %50 ila %90 arasında olduğu belirlenmiştir. Yalnızca bir B. spicifera izolatı bitkilerde zayıf patojen olarak belirlenmiş, B. australiensis izolatları hiçbir hastalık belirtisi oluşturmamıştır. B. sorokiniana bitkilerin yaş ağırlıklarında kontrollere oranla önemli düşüşlere yol açarken, B. spicifera'nın dört izolatının bitki ağırlığında artış sağladığı belirlenmiştir. Sonuç olarak, tüm agro-ekolojik bölgelerdeki B. sorokiniana izolatlarının şiddetli kök çürüklük hastalığı yaptığı ve bitki ağırlığında önemli miktarda azalmalara neden olduğu belirlenmiştir. Diğer taraftan, B. spicifera izolatlarının buğday bitkilerinin köklerinde zayıf hastalık belirtileri oluşturduğu, B. australiensis'in ise bitkilerin köklerinde hastalığa yol açmadığı ve bitki ağırlığını olumsuz olarak etkilemediği görülmüştür.
Fusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR) in cereals worldwide. Since F. culmorum has a great level of genetic diversity and the parasexual stage is assumed for this phytopathogen, characterization of isolates from different regions is significant step in food safety and controlling the HB. In this study, it was aimed to characterize totally 37 F. culmorum isolates from Turkey via sequence related amplified polymorphism (SRAP) marker based genotyping. MAT-1/MAT-2 type assay was also used in order to reveal intraspecific variation in F. culmorum. MAT-1 and MAT-2 specific primer pairs for mating assays resulted in 210 and 260 bp bands, respectively. 11 of isolates were belonged to MAT-1 type whereas 19 samples were of MAT-2. Remaining 7 samples yielded both amplicons. Totally 9 SRAP primer sets yielded amplicons from all isolates. Genetic similarity values were ranged from 39 to 94.7%. Total band number was 127 and PCR product sizes were in the range of 0.1-2.5 kb. Amplicon numbers for individuals were ranged from 1 to 16. According to data obtained from current study, SRAP based genotyping is powerful tool for supporting the data obtained from investigations including phenotypic and agro-ecological characteristics. Findings showed that SRAP-based markers could be useful in F. culmorum characterization.
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