Candida albicans is the causative agent of fatal systemic candidiasis. Due to limitations of antifungals, new drugs are needed. The anti-virulence effect of plant essential oils (EOs) was evaluated against clinical C. albicans isolates including cinnamon, clove, jasmine and rosemary oils. Biofilm, phospholipase and hemolysin were assessed phenotypically. EOs were evaluated for their anti-virulence activity using phenotypic methods as well as scanning electron microscopy (SEM) and atomic force microscopy (AFM). Among the C. albicans isolates, biofilm, phospholipase and hemolysins were detected in 40.4, 86.5 and 78.8% of isolates, respectively. Jasmine oil showed the highest anti-biofilm activity followed by cinnamon, clove and rosemary oils. SEM and AFM analysis showed reduced adherence and roughness in the presence of EOs. For phospholipase, rosemary oil was the most inhibitory, followed by jasmine, cinnamon and clove oils, and for hemolysins, cinnamon had the highest inhibition followed by jasmine, rosemary and clove oils. A molecular docking study revealed major EO constituents as promising inhibitors of the Als3 adhesive protein, with the highest binding for eugenol, followed by 1,8-cineole, 2-phenylthiolane and cinnamaldehyde. In conclusion, EOs have a promising inhibitory impact on Candida biofilm, phospholipase and hemolysin production, hence EOs could be used as potential antifungals that impact virulence factors.
Lavender is a good source of essential oils and phenolic metabolites for food, medicine, and cosmetic applications. Due to cross-pollination, lavender has substantial plant to plant variation and therefore a high degree of genetic inconsistency in the level of phytochemicals produced for diverse applications. Tissue culture methods, using benzyladenine-induced shoot organogenesis, were used to isolate clonal lines originating from individual heterozygous seeds among a heterogeneous seed population to exploit the genetic heterogeneity. Subsequently, in a two-step method, clonal shoots of each clonal line were evaluated for the ability to tolerate Pseudomonas inoculation and various levels (0-200 microM) of proline analogue, azetidine-2-carboxylate. On the basis of tolerance to Pseudomonas and proline analogue treatments, multiple shoot forming ability, biomass, rosmarinic acid, total phenolics, and total chlorophyll, 20 separate clonal lines were screened and isolated for further vegetative propagation and evaluation. From the clonal lines isolated, lines LH-14, LH-15, LH-17, and LH-11 showed the best potential for overexpression of phenolic metabolites in response to Pseudomonas and proline analogue.
1999) Screening of high biomass and phenolic producing clonal lines of spearmint in tissue culture using pseudomonas and azetidine-2 carboxylate., Food Biotechnology, 13:3, 227-253,
AbstractRosmarinic acid (RA) and related phenolics are natural antioxidants found as secondary metabolites in spearmint (Mentha spicata). These phenolic-secondary metabolites have diverse food processing and nutraceutical applications. Since natural cross-pollination results in plant to plant variation in the level of phenolic metabolites, tissue culture-based techniques are essential to isolate elite antioxidant-producing clonal lines. The objective of this research is to develop tissue culture-based selection techniques to isolate high rosmarinic acid and phenolic-producing clonal lines from a heterogenous bulk seed population of spearmint. Multiplied clonal shoots of each line were screened for tolerance to azetidine-2-carboxylate (A2C). Individual shoot apex of each line were also screened for Pseudomonas tolerance. Rosmarinic acid and total phenolics were assayed in 4 Corresponding author
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections because of its high resistance. Here, we study the antibiotic resistance in MRSA clinical isolates and their relation to integron I occurrence. A total of 88 clinical Staphylococcusaureus isolates were collected. MRSA were identified by the disk diffusion method (DDM) and confirmed by PCR, and antibiogram was determined by DDM. Integron I, II and the aacA4 gene were investigated by PCR. Integrase-positive strains were analyzed for the presence of resistance gene cassettes by sequencing. All isolates were identified as MRSA by DDM and confirmed by PCR. All isolates were resistant to ampicillin and cefoxitin. Concerning aminoglycosides, the frequency of resistance was reported for streptomycin (60.7%), tobramycin (37.1%) gentamicin (36%), and for amikacin (15.9%). Integron I was detected in 41 isolates (46.6%), while integron II was detected in three isolates (3.4%). Sequencing of the integron I-cassette indicated the exclusive prevalence of addA gene variants mediating aminoglycoside resistance. The aacA4 gene was found in DNA of 31 isolates (35.22%). This study revealed the high existence of MRSA. Furthermore, the AacA4 gene and class I integron harboring aadA gene were predominant in MRSA isolates.
Soil hosts myriads of living organisms with the extensive potential to produce bioactive compounds. Bacteria are the major soil inhabitants that represent a rich reservoir for antibiotic production along with their role in recycling nutrients and maintenance of the soil ecosystem. Here, from 55 tested soil samples, we isolated and identified a novel antibiotic-producing bacterial strain with a phylogenetically closest match to Bacillus subtilis sp. based on BLASTN search of GenBank for the 16S rRNA gene sequence. We characterized this novel strain through microscopic, biochemical, and molecular techniques, combined with testing its potential antimicrobial activity. Chemical studies revealed that the antibiotic produced by this strain is a glycopeptide. It exhibited profound activity against both methicillin-resistant Staphylococcus aureus (MRSA) and Candida albicans. The antibiotic is optimally produced at 37 °C after 28 h of growth. The biocompatibility of the extracted antibiotic was tested over a wide range of factors including temperature, pH, surfactants, and metal salts. To confirm its therapeutic potential, a sterile solution of the antibiotic was tested in vivo against bacteria-induced keratitis in rats where significant healing activity was recorded. Hence, this soil Bacillus strain may lead to the development of novel antibiotics for the treatment of human pathogens.
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