Escherichia coli O157:H7 has been reported as
an important pathogenic bacteria causing serious infection and economic
loss. However, detection of Escherichia coli O157:H7
needs improvement, given its current complexity and sensitivity. Herein,
we attempt to build a fluorescence sensing method to detect Escherichia coli O157:H7 with easy operation and high efficiency.
The target virulence gene sequences are recognized and cleaved by
the CRISPR-Cas9 system, and trigger strand displacement amplification
and rolling circle amplification. After amplification reactions, massive
products can hybridize with the probes, the fluorescence of which
are quenched based on a metal–organic framework platform, leading
to the fluorescence recovery at typical excitation/emission wavelengths
of 480/518 nm. This method exhibits high sensitivity with the detection
limit at 4.0 × 101 CFU mL–1 and
a wide range from 1.3 × 102 CFU mL–1 to 6.5 × 104 CFU mL–1. Meanwhile,
this assay also shows significant specificity and applies to practical
samples with high accuracy. Therefore, our method would have great
potential application in bacterial detection, food safety monitoring,
or clinical diagnostics.
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