Effects of bisphenol A and nanoscale and microscale polystyrene plastic exposure on particle uptake and toxicity in human Caco-2 cells

Wang, Qiangqiang; Bai, Jintao; Ning, Baoan; Fan, Longxing; Sun, Tieqiang; Fang, Yanjun; Wu, Jin; Li, Shuang; Duan, Chenhui; Zhang, Yingchun; Liang, Jun; Gao, Zhixian

doi:10.1016/j.chemosphere.2020.126788

Cited by 149 publications

(59 citation statements)

References 38 publications

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“…This pathway is important for inflammatory diseases and immune response. 15,20,21 It is involved in several immune response processes, including malignant transformation, the development and survival of lymphocytes and lymphoid organs, and overall control of the immune response. 22 A series of immunoregulation processes of antigen entering Caco-2 cells, such as activation of NF signaling pathway and release of inflammatory factors are shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

Effect of roasted peanut allergen Ara h 3 protein on the sensitization of Caco‐2 cells

et al. 2021

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BACKGROUND: Roasted peanut is widely loved as a kind of food with rich taste. However, peanut allergy is one of the major threats to human health, which affects about 5% of children and 1.4-2% of adults in the world. RESULTS: To evaluate the sensitization mechanism of peanut allergen Ara h 3, Caco-2 cells as the model, which has the similar structure and function to differentiated small intestinal epithelial cells. Compared with Ara h 3-raw (purified from raw peanut) group, more significant results such as the inhibited Caco-2 cell viability and proliferation, the increased secretion of reactive oxygen species (ROS) and the decreased transepithelial electrical resistance were obtained in Ara h 3-roasted (purified from roasted peanut) group. Accordingly, oxidative stress and NF-κB signaling pathway were more imbalanced, which lead to the increased of thymic stromal lymphopoietin (TSLP), interleukin 6 (IL-6), IL-8 and monocyte chemotactic protein 1 (MCP-1). Then, the gene expression of tight junction proteins ZO-1, occludin and JAM-1 were reduced, which proved that the integrity of the Caco-2 monolayer barrier is severely damaged.CONCLUSION: These finding identify the mechanisms of the allergenicity of roasted peanut allergy proteins are probably associated with intestinal uptake and cytokine dependent allergies. The aggravated allergic reaction might be caused by the increment of TSLP, IL-6, IL-8 and MCP-1 due to the activated NF-κB signaling pathway, and the enhanced transport of Ara h 3-roasted protein by Caco-2 monolayer.

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Section: Introductionmentioning

confidence: 99%

Effect of roasted peanut allergen Ara h 3 protein on the sensitization of Caco‐2 cells

et al. 2021

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“…The absence of a dose-response correlation of ROS production is not an abnormal result as other studies using PS-MPs while showing significant differences compared to the control cells, highlighted no significant differences with respect to concentration [ 57 , 63 , 64 ]. It is plausible to believe that the absence of a dose-effect response is attributed to the rapidity with which the cells, depending on the stimulus undergone, counteract the pro-oxidant effect.…”

Section: Discussionmentioning

confidence: 56%

Acute and Sub-Chronic Effects of Microplastics (3 and 10 µm) on the Human Intestinal Cells HT-29

Visalli

Facciolà

Ciarello

et al. 2021

IJERPH

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Due to ingestion of contaminated foods, the human gastrointestinal tract is the most likely site of exposure to microplastics (MPs) with gut barrier dysfunction and intestinal inflammation. Aimed to assess the effects induced by MPs with different granulometry (polystyrene (PS) 3 and 10 µm), we performed an in vitro study by using the human intestinal cell line HT29. As a novelty, we assessed the sub-chronic exposure extending the treatment up to 48 days simulating the in vivo situation. In the range of 100–1600 particles mL−1, both the PS suspensions had moderate cytotoxicity after 24 h with percentages of mortality between 6.7 and 21.6 for the 10 µm and 6.1 and 29.6 for the 3 µm PS. Microscopic observation highlighted a more pronounced lysosomal membrane permeabilization in HT29 exposed to PS 3µm. Reactive oxygen species production was higher in cells exposed to PS 10 µm, but sub-chronic exposure highlighted the ability of the cells to partially neutralize this effect. Comet-assay confirmed the temporary oxidative damage that was PS-induced. Overall, considering the very fast turnover of intestinal cells, the increase in cell mortality, equal to 25% and 11% for 3 and 10 µm PS-MPs for each time point, could trigger intestinal disorders due to prolonged exposure.

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“…They found that polystyrene particles caused higher oxidative stress than polyethylene particles and they attributed this to the smaller size of polystyrene compared to polyethylene particles. Wang et al (2020) observed increasing uptake rates of polystyrene particles from colonic cancer Caco-2 cells with decreasing particle size. The uptake rates of nanoplastics were 73%, and 71% for 300 nm and 500 nm sized polysterene particles respectively.…”

Section: Health Impact From Mps Inhalationmentioning

confidence: 79%

Overview on the occurrence of microplastics in air and implications from the use of face masks during the COVID-19 pandemic

Torres-Agullo

Karanasiou

Moreno

et al. 2021

Science of The Total Environment

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Environmental pollution from microplastics (MPs) in air is a matter of growing concern because of human health implications. Airborne MPs can be directly and continuously inhaled in air environments. Especially high MPs contributions can be found in indoor air due to the erosion and breakage of consumer, domestic and construction products, although there is little information available on their sources and concentrations and the risks they might pose. This is in part due to the fact that sampling and analysis of airborne MPs is a complex and multistep procedure where techniques used are not yet standardized. In this study, we provide an overview on the presence of MPs in indoor air, potential health impacts, the available methods for their sampling and detection and implications from the use of face masks during the COVID-19 pandemic.

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Effects of bisphenol A and nanoscale and microscale polystyrene plastic exposure on particle uptake and toxicity in human Caco-2 cells

Cited by 149 publications

References 38 publications

Effect of roasted peanut allergen Ara h 3 protein on the sensitization of Caco‐2 cells

Effect of roasted peanut allergen Ara h 3 protein on the sensitization of Caco‐2 cells

Acute and Sub-Chronic Effects of Microplastics (3 and 10 µm) on the Human Intestinal Cells HT-29

Overview on the occurrence of microplastics in air and implications from the use of face masks during the COVID-19 pandemic

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