Epigenetic changes such as DNA methylation regulate gene expression in normal development. Methotrexate and Adriamycine are antineoplastic drugs that target DNA and enzymes acting on DNA. We aimed to evaluate their cytotoxic effect on cell lines and on female mice to investigate the in vivo influence of both drugs on the DNA methylation and subsequently the protein expression. The total level of DNA methylation showed a significant reduction from 62.2% to 36.7%, 36.6% as compared to control group, when using different doses of MTX and ADR. Hepatic protein pattern revealed five bands with low MW (16 -6.1 KDa) in acute and LD50 doses. In conclusion DNA methylation is influenced by anticancer drugs in a dose-dependent manner. Some specific protein fragments may be considered as a potential markers associated with high dose of anticancer drugs.

Impairment of spermatogenesis has been shown in patients treated with chemotherapy against different malignancies resulting in azoospermia. The present study was designed to assess the ability of anti-SCa-1and CD105 mesenchymal stem cells to restore fertility in induced azoospermic mice following cyclophosphamide administration. Forty male mice were divided randomly into four groups, each group included 10 male albino mice which has an average body weight 22-25 g; group (1): normal control, group (2): male azoospermic mice induced with cyclophosphamide, group (3): male azoospermic mice received anti-SCa-1 + stem cells into caudal vein, group (4): male azoospermic mice received CD105 MSCs into caudal vein. Androgen hormonal profile and histological assessment of testicular tissues were evaluated pre-and post-treatment with anti-Sca-1 + and mesenchymal stem cell transplantation. Results showed that male azoospermic mice treated with anti-SCa-1+ and CD105 MSCs stem cells after 12 weeks of treatment are able to improve androgen hormonal profile levels and resuming spermatogenesis as verified by histological assessment of testicular tissues and hormonal profile as compared to control groups. Also data obtained from the study showed no significant differences between anti-SCa-1+ and CD105 MSCs treated groups. In conclusion, both of anti-SCa-1 and CD105 MSCs have the potentiality to differentiate into germ cells and sperms in vivo in testicular microenvironment, and also able to restore testicular functions.

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The present study aimed to evaluate the antitumor activity of newly synthesized oxidized form of sodium hypochlorite. Different cell lines were susceptible to Sodium hypochlorite cytotoxicity at acute, LD 50 and therapeutic doses of NaOCl. Therapeutic dose of sodium hypochlorite modulated the significantly reduced level of Hb, IL-12, TNF and the increased levels of creatinine, GPT and GOT towards the normal values. The histopathological study of liver, kidney and spleen groups showed a very significant improvement compared to normal and positive control groups. Sodium Hypochlorite exhibited an anticancer effect on different cell lines in vitro and against EAC in mice.

Objective: to evaluate the stem cell in treating renal diseases. To evaluate the effect of stem cells on renal cell apoptosis and necroptosis. Background: The use of stem cells is the hope for all patients with end-stage renal diseases. We isolate stem cells from umbilical cord blood to treat renal diseases by using the Vero cell line as a renal cell which is treated by hydrogen peroxide as renal failure. P53, RIPK1, and EGFR genes were detected by RT PCR to show the improvement by using stem cell therapy for renal diseases. Mesenchymal stem cells are the future hope for the treatment of renal diseases associated with renal cell apoptosis and necroptosis. Methodology: Mesenchymal CD105 Stem Cells Separation from Umbilical Cord Blood from 20 pregnant females. By forming buffy coat mononuclear leukocytes. Magnetic labeling of CD105 and Separation to stem cells. stem cell proliferation by using Dulbecco Modified Eagle medium (DMEM). After proliferation passages 1, 2, and 3 freeze cells at -20c. For one week then thawing at room temperature to make stem cell extraction. Vero cell line treated by H2O2 1.6 mm for 5 hours to reach sub-lethal. The cytotoxicity of the cell was caused by hydrogen peroxide measured by MTT assay and Spectrophotometry. The Vero cell line is divided into 4 groups group1: normal control, group 2: sub-lethal, group 3: sub-lethal treated by stem cell extraction, and group 4: sub-lethal treated by stem cells. P53, RIPK1, and EGFR genes were detected in 4 groups by RT PCR. Results: P53, RIPK1, and EGFR levels showed a highly significant difference among studied groups with elevated levels of P53 and RIPK1 and reduced levels of EGFR in the sub-lethal renal cell group with an improvement of cells treated with mesenchymal stem cells. Mesenchymal stem cells showed better results when compared with mesenchymal stem cell extract. Conclusion: Mesenchymal stem cells demonstrated a good effect on renal cell line injury, apoptosis, and necroptosis.