fertility in induced azoospermic mice

Abstract

Impairment of spermatogenesis has been shown in patients treated with chemotherapy against different malignancies resulting in azoospermia. The present study was designed to assess the ability of anti-SCa-1and CD105 mesenchymal stem cells to restore fertility in induced azoospermic mice following cyclophosphamide administration. Forty male mice were divided randomly into four groups, each group included 10 male albino mice which has an average body weight 22-25 g; group (1): normal control, group (2): male azoospermic mice induced with cyclophosphamide, group (3): male azoospermic mice received anti-SCa-1 + stem cells into caudal vein, group (4): male azoospermic mice received CD105 MSCs into caudal vein. Androgen hormonal profile and histological assessment of testicular tissues were evaluated pre-and post-treatment with anti-Sca-1 + and mesenchymal stem cell transplantation. Results showed that male azoospermic mice treated with anti-SCa-1+ and CD105 MSCs stem cells after 12 weeks of treatment are able to improve androgen hormonal profile levels and resuming spermatogenesis as verified by histological assessment of testicular tissues and hormonal profile as compared to control groups. Also data obtained from the study showed no significant differences between anti-SCa-1+ and CD105 MSCs treated groups. In conclusion, both of anti-SCa-1 and CD105 MSCs have the potentiality to differentiate into germ cells and sperms in vivo in testicular microenvironment, and also able to restore testicular functions.

Fertility Protection, A Novel Concept: Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Protect against Chemotherapy-Induced Testicular Cytotoxicity

“…These reports highlight the regenerative abilities of MSCs (i.e., anti-apoptotic, anti-inflammatory, anti-oxidative stress, DNA repair enhancement) that might contribute to their ferility restoration abilities. However, authors also believe that MSCs could potentially differentiate into germ cells and sperm based on the in vivo testicular microenvironment and hence restore testicular functions in that way [ 64 ]. However, the present study clearly suggests the absence of this differentiation phenomenon altogether.…”

Therapeutic Effect of Murine Bone Marrow-Derived Mesenchymal Stromal/Stem Cells and Human Placental Extract on Testicular Toxicity Resulting from Doxorubicin in Rats

“…The mechanisms involved in the restorative effects of MSCs on testicular function are due to the ability of transplanted MSCs to stimulate the production of substances capable of inhibiting ROS, cell death, inflammation, and mutagenic activities [ 58 ]. MSCs were able to differentiate into spermatocytes to release hormones [ 59 ]. On the other hand, MSCs play a role in restoring spermatogenesis by differentiating into sperm cells or maintaining spermatogonial stromal cells.…”

“…These results are indicative of the therapeutic effect of MSC or HPE against doxorubicin gonadotoxicity. Earlier studies recorded that MSC treatment resulted in a rapid reversal of testicular injury and marked restoration of normal histological structure of testes after sterility induced by DOX [ 59 ]. The effect of MSCs is essentially happening through direct recovery of injured organs and cell development; this was manifested by the presence of MSCs in the interstitial connective tissue between the seminiferous tubules, and their morphology was like that of Leydig cells [ 60 ], or the effect is happening through indirect inducement of cell proliferation through paracrine signaling, which includes production of growth factors that help in interactions between cells [ 61 ].…”