Background
The mortality and morbidity associated with oral squamous cell carcinoma (OSCC) can be greatly reduced if tumor markers which can detect OSCC at an early stage are available. The use of saliva as an alternative to blood could provide a substantial advantage in sampling convenience. Cancer antigen 125 (CA 125) is a tumor-associated antigen found to be increased in epithelial tumors like oral, breast and ovarian cancers.
Aim
To determine whether salivary CA 125 levels are increased significantly in OSCC patients than the control group.
Materials and methods
Sixty OSCC patients and 60 healthy controls were taken for the study. Saliva samples from both the groups were collected, centrifuged and supernatant fluid were subjected to ELISA for assessment of CA 125. The mean salivary CA 125 values of OSCC patients and control group were statistically analyzed using Mann-Whitney U-test.
Results
The mean salivary CA 125 concentration of OSCC group was 320.25 and that of control group was 33.14. Thus, CA 125 was found to be significantly increased in the saliva of OSCC patients than the control group (p < 0.001). Also, there was significant increase in the CA 125 levels as the stage of OSCC increased.
Conclusion
The convenience, reliability and noninvasive nature of salivary CA 125 testing makes it a feasible adjunctive diagnostic tool for detection of OSCC.
How to cite this article
Balan JJ, Rao RS, Premalatha BR, Patil S. Analysis of Tumor Marker CA 125 in Saliva of Normal and Oral Squamous Cell Carcinoma Patients: A Comparative Study. J Contemp Dent Pract 2012;13(5):671-675.
Clear cells are routinely encountered in the histopathological sections. They most frequently result from fixation artefacts; cytoplasmic accumulation of water, glycogen, lipids, mucins; hydropic degeneration of organelles, etc. When these clear cells predominate in a tumor, arriving at a definitive diagnosis becomes problematic. Thus, this review gives an idea of clear cells associated with various conditions, causes for clearing of these cells, clear cell tumors of the head and neck and a systematic approach towards arriving at an appropriate diagnosis of these tumors.
How to cite this article
Premalatha BR, Rao RS, Patil S, Neethi H. Clear Cell Tumors of the Head and Neck: An Overview. World J Dent 2012;3(4):344-349.
Background:Xylene is used as a clearing agent in hematoxylin and eosin (H and E) staining of tissue sections in routine histopathology based diagnosis. However, the hazards associated with exposure to xylene are of concern. Numerous solutions mainly essential oils have been evaluated in the past as clearing agents, which can possibly be substituted for xylene during the routine tissue processing.Aim:The aim of this study is to compare the efficacy of essential oil (cedarwood oil), as a possible replacement for xylene in H and E staining procedures.Materials and Methods:The study was carried out in the Department of Oral Pathology and Microbiology. Thirty paraffin blocks of the routine biopsy specimen were retrieved from the department archives. The cedarwood oil was procured from organic and essential oil dealer in the local market. Two to three paraffin sections of four micron thickness were cut from each of the 30 paraffin blocks of processed tissue specimens, were subjected to different clearing agents: Essential oil (8% cedarwood oil) or xylene and stained with H and E stain. The stained sections were scored based on nuclear and cytoplasmic details, clarity and uniformity of staining.Results:Significant correlation was observed between cedarwood oil and xylene in terms of the three staining quality parameters assessed.Conclusions:We conclude that cedarwood oil can be an effective, eco-friendly and safe alternative to xylene as a clearing agent in the histopathological laboratory.
Calcifying cystic odontogenic tumor (CCOT) is a rare lesion accounting for only 2% of all odontogenic cysts and tumors. CCOTs can occur alone or in association with other odontogenic tumors such as odontomas. Pigmented intraosseous odontogenic lesions are rare. Among them, pigmented CCOT is known to occur with greater frequency. Only six cases of combination of pigmentation CCOT associated with odontoma have been reported in the literature. We herein present such a rare case occurring in the maxillary anterior region in a 13-year-old female patient.
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