Comparative efficacy of cedarwood oil and xylene in hematoxylin and eosin staining procedures: An experimental study

Indu, Sudip; Ramesh, V.; Indu, Priyanka Chakravarty; Prashad, Karthikshree V; Premalatha, B R; Ramadoss, K

doi:10.4103/0976-9668.136167

Cited by 15 publications

(15 citation statements)

References 4 publications

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“…Cedar wood oil and Coconut oil were less effective to assess cellular architecture compared to Xylene and Dish wash liquid. This was not in accordance with study conducted by Sudip Indu et11 12…”

contrasting

confidence: 75%

A Comparative Study of the Efficacy Of Cedarwood Oil, Coconut Oil and Dish Wash Liquid as Alternatives to Xylene As Deparaffinizing Agents

Sithara¹,

B²,

Desai³

2021

PIJR

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Xylene is an aromatic hydrocarbon which is widely used as a deparaffinizing agent, and it is extremely biohazardous. Various biosafe alternatives to xylene have been studied in the past with variable results. The aim of this study was to compare the efficacy of Cedar wood oil, Coconut oil and Dish wash liquid (DWL) with Xylene as a deparaffinizing agent and to figure out the best biosafe alternative to Xylene. The study consisted of 50 samples and results were analyzed based on the cellular architecture and total quality of staining. Xylene and DWS showed same result of high-quality staining in case of cellular architecture. But in case of total quality of staining, only Xylene showed the best results. Hence, we could conclude that though Xylene is toxic, it is still the best deparaffinizing agent and more studies has to be done in this field to prove the efficiency of natural agents.

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confidence: 75%

A Comparative Study of the Efficacy Of Cedarwood Oil, Coconut Oil and Dish Wash Liquid as Alternatives to Xylene As Deparaffinizing Agents

Sithara¹,

B²,

Desai³

2021

PIJR

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“…Cedarwood oil at 8%, being nontoxic, noninflammable, nonhazardous, and easy to handle, produced good quality staining with sufficient clarity and uniformity of staining in comparison with xylene. [12]…”

Section: Discussionmentioning

confidence: 99%

A substitute to xylene in deparaffinization and clearing prior to coverslipping in histopathology

Alwahaibi

Aldughaishi

2019

J Lab Physicians

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INTRODUCTION: Deparaffinization and clearing prior to coverslipping are important steps in all staining methods in histopathology. Xylene is the most commonly used agent worldwide. However, xylene is toxic. We evaluated safer alternative dewaxing and clearing agents prior to coverslipping in a histopathology laboratory. MATERIALS AND METHODS: Thirteen different fresh surgical tissues were cut into two halves. One half processed using xylene and the other half processed using UltraClear™. Five groups were designed. For each Group of A, B, C, and D, 100 slides were cut from xylene-processed blocks. For Group E, 100 slides were cut from UltraClear™-processed blocks. Group A is the standard method. Group B evaluates UltraClear™ as a dewaxing agent only. Group C evaluates UltraClear™ as a clearing agent prior to coverslipping only. Group D evaluates UltraClear™ as both dewaxing and clearing agents prior to coverslipping. Group E evaluates UltraClear™ as both dewaxing and clearing agents prior to coverslipping. Six parameters were evaluated: nuclear staining, cytoplasmic staining, cell morphology, clarity of staining, uniformity of staining, and cost. RESULTS: Groups B, C, and D showed 79% (P = 0.054), 83% (P = 0.221), and 80% (P = 0.079) adequacy when compared with Group A (89%), respectively. However, Group E showed only 76% (P = 0.016) adequacy. UltraClear™ is more expensive than xylene. CONCLUSION: UltraClear™ is a promising dewaxing agent. It is also a good clearing agent for use prior to coverslipping in histopathology laboratory. Cost-benefit balance between safety of laboratory workers, good quality staining, and cost-effective strategy needs to be further studied.

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“…used 8% cedarwood oil to produce quality staining with sufficient clarity and uniformity. [ 16 ] Pandey et al . used DWS as an alternative to xylene and alcohol in H and E staining and found that 84% sections which were routinely stained with xylene and 84% sections with liquid DWS were adequate for diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of biosafe alternatives as xylene substitutes in hematoxylin and eosin staining procedure: A comparative pilot study

Sravya

Guttikonda

Kumari

et al. 2018

J Oral Maxillofac Pathol

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Comparative efficacy of cedarwood oil and xylene in hematoxylin and eosin staining procedures: An experimental study

Cited by 15 publications

References 4 publications

A Comparative Study of the Efficacy Of Cedarwood Oil, Coconut Oil and Dish Wash Liquid as Alternatives to Xylene As Deparaffinizing Agents

A Comparative Study of the Efficacy Of Cedarwood Oil, Coconut Oil and Dish Wash Liquid as Alternatives to Xylene As Deparaffinizing Agents

A substitute to xylene in deparaffinization and clearing prior to coverslipping in histopathology

Evaluation of biosafe alternatives as xylene substitutes in hematoxylin and eosin staining procedure: A comparative pilot study

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