A substitute to xylene in deparaffinization and clearing prior to coverslipping in histopathology

Alwahaibi, Nasar; Aldughaishi, Sirin Hamed

doi:10.4103/jlp.jlp_169_18

Cited by 7 publications

(5 citation statements)

References 12 publications

(19 reference statements)

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“…The mixture is used in hand washing of cutlery. The concept of using dish wash liquid as dewaxing agent is because of its surfactant property reducing the surface tension [ 11 ]. Some have highlighted the advantages of hot dishwashing soap (DWS) solution for deparaffinization of tissue sections for H&E staining and stated that even in special staining procedures like periodic acid-Schiff (PAS) staining and Van Gieson staining it can be applied.…”

Section: Resultsmentioning

confidence: 99%

A comparative study of efficacy of coconut oil, lemon water and dishwashing liquid as surrogates to xylene

Thajudeen

Raghavan

Govindarajan

et al. 2022

Environ Anal Health Toxicol

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Histopathology is the field of science that helps in analyzing the architectural pattern of cells under the microscope. Hematoxylin and eosin-stained sections are used for routine histopathological examination. Xylene being a biohazardous hydrocarbon is used in many steps of tissue processing and laboratory personnel are exposed to this toxic substance. Maximum exposure to xylene occurs in the step of deparaffinization, for which alternate safer methods should be introduced. This study compares the efficacy of natural products like coconut oil, lemon water, less chemical substance like dish wash liquid with xylene as deparaffinizing agent. 50 paraffin embedded sections were used in each of the groups using xylene, coconut oil, diluted lemon water and dish wash liquid as deparaffinizing agents. 80% of slides using dishwashing liquid, 64% using lemon water and 42% of slides using coconut oil showed excellent cellular features. 96% of slides using xylene showed good quality staining, 54% of slides using dishwashing liquid and 40% slides using lemon water showed good quality staining. Only 4% of slides prepared using coconut oil showed good quality staining. Dishwashing liquid is the best surrogate and among the natural products, diluted lime water yields a better result and coconut oil, the least productive as deparaffinizing agent in this study.

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Section: Resultsmentioning

confidence: 99%

A comparative study of efficacy of coconut oil, lemon water and dishwashing liquid as surrogates to xylene

Thajudeen

Raghavan

Govindarajan

et al. 2022

Environ Anal Health Toxicol

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“…While the grinding of the embedded samples into small pieces was a procedure in common with similar toxicological research articles [11,12], the solubilization of the paraffin wax embedding the samples was a more delicate procedure. Classic deparaffinization involving the use of xylene or a xylene substitute and subsequent rehydrating passages in a decreasing alcoholic ladder [17] was considered as too invasive under a toxicological point of view; the washing out and subsequent loss of analytes had to be taken into consideration, and we therefore decided to proceed by solubilizing paraffin in a non-polar solvent n-hexane. Analytes of toxicological interest are polar molecules and we therefore decided to proceed with a liquid-liquid extraction using acetonitrile.…”

Section: Discussionmentioning

confidence: 99%

Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago

et al. 2022

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Purpose Since the solely certain remnants of a performed autopsy are formalin-fixed paraffin-embedded (FFPE) samples, stored in the archives of every institute of legal medicine, we managed to extract molecules of toxicological interest from these specimens. Methods We assessed the analysis of ten fresh liver samples collected from heroin-related deaths and then histologically processed the same samples. The embedded blocks were then extracted by means of a new extracting method and the eluates were measured. We also selected five toxicological cases of heroin-related fatalities that were examined 20 years ago, collected the toxicological result documents of the analysis that were carried out at the time and then processed the corresponding FFPE liver samples that were stored in the archives. Results We managed to isolate heroine-related metabolites from 20-year-old paraffin-embedded blocks and calculated ratios to evaluate the performance of our new extraction. Conclusions According to our study, it is feasible to carry out a toxicological examination on old histological samples and, therefore, this matrix can be considered as a new alternative specimen for chemical-analytical evaluations of past cases or when fresh samples are not available anymore. The new extractive method was evaluated as efficient in treating these complex, paraffin-embedded samples. It was surprising that the target compounds could be quantitated from FFPE bocks created as long as 20 years ago.

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“…Di laboratorium histologi, pekerja laboratorium medis khususnya petugas histologi yang bertugas dalam mewarnai jaringan memiliki resiko yang berbahaya baik bahaya kimiawi, biologis, mekanis maupun lingkungan. Apabila tenaga medis terutama terus terus menghirup xilol atau bekerja dengan zat zat yang berbahaya seperti xilol secara terus menerus dalam waktu yang lama, hal ini bisa berdampak buruk bagi kesehatan tubuh manusia tubuh termasuk cedera jantung dan ginjal, diskrasia darah yang fatal, eritema kulit, dan sekunder infeksi dan sebagainya Alwahaibi and Aldughaishi (2019). Paparan uap xilol cepat diserap melalui paru-paru dan secara perlahan melalui kulit.…”

Section: Pendahuluanunclassified

“…Dalam penelitian ini menjelaskan bahwa sabun pencuci piring cair dapat menganggantikan xilol. Sabun cuci piring tidak beracun, murah, mudah ditangani, dan dibuang bisa dijadikan alternatif yang efektif untuk xilol Alwahaibi and Aldughaishi (2019).…”

Section: Hasil Dan Pembahasanunclassified

Utilization Dishwashing Soap as a Subtitute of Xylol in the Deparaffinization process of Hematoxylin-Eosin Dye: Review Article

Mamay

Mutmaina

Nurahma

2022

medicra

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Deparaffinization in the hematoxylin eosin staining process aims to remove paraffin from the tissue, clean the tissue and maximize dye absorption. Utilization xylol as deparaffinization has a drawback, if inhaled too much it will cause harm to the health of the laboratory staff. Considering this, a safer alternative to xylol is needed, one of which is dish washing soap. This study was conducted to determine the use of liquid dish soap at the deparaffinization stage on the quality of tissue staining results. The research method in this research is a literature review study conducted based on sources from several articles published digitally in the Science Direct, Pub Med, NCBI, Researchgate, Google Scholar with the keywords deparafinization, xylene, dish washing soap and Hematoxylin-eosin. Based on the results of a review of ten journals, the concentration of dishwashing soap used was 1.5%, 1.7%, 2% and 2.5% with the most widely used concentration of 1.7%. The most used deparaffinization time with dish soap was 1 minute, one study used 2 minutes. Of the several differences in deparaffinization time and concentration of dish soap, the results adequacy of nuclear and cytoplasmic staining, clarity, uniformity and crispness of staining were very good in almost all studies, there was no difference in quality with the use of xylol. So it can be concluded that the use of dish soap in the deparaffinization process for Hematoxylin staining showed good staining of all tissues. Dish soap can be used as an alternative agent in deparaffinization of Hematoxylin-Eosin staining.

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A substitute to xylene in deparaffinization and clearing prior to coverslipping in histopathology

Cited by 7 publications

References 12 publications

A comparative study of efficacy of coconut oil, lemon water and dishwashing liquid as surrogates to xylene

A comparative study of efficacy of coconut oil, lemon water and dishwashing liquid as surrogates to xylene

Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago

Utilization Dishwashing Soap as a Subtitute of Xylol in the Deparaffinization process of Hematoxylin-Eosin Dye: Review Article

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