The Pelger-Huët anomaly (PHA), an autosomal dominant condition ,is characterized by morphologically abnormal neutrophil. This article summarizes the history of PHA , pathogenesis and guidance for differentiating congenital or acquired PHA, with their different morphological variants. We report an interesting case of pseudo-PHA in a 70 year old male diagnosed with Leptospirosis, showing unilobed, unilobed intended, bilobed pelgeroid cells in the peripheral smear.
Histopathology is the field of science that helps in analyzing the architectural pattern of cells under the microscope. Hematoxylin and eosin-stained sections are used for routine histopathological examination. Xylene being a biohazardous hydrocarbon is used in many steps of tissue processing and laboratory personnel are exposed to this toxic substance. Maximum exposure to xylene occurs in the step of deparaffinization, for which alternate safer methods should be introduced. This study compares the efficacy of natural products like coconut oil, lemon water, less chemical substance like dish wash liquid with xylene as deparaffinizing agent. 50 paraffin embedded sections were used in each of the groups using xylene, coconut oil, diluted lemon water and dish wash liquid as deparaffinizing agents. 80% of slides using dishwashing liquid, 64% using lemon water and 42% of slides using coconut oil showed excellent cellular features. 96% of slides using xylene showed good quality staining, 54% of slides using dishwashing liquid and 40% slides using lemon water showed good quality staining. Only 4% of slides prepared using coconut oil showed good quality staining. Dishwashing liquid is the best surrogate and among the natural products, diluted lime water yields a better result and coconut oil, the least productive as deparaffinizing agent in this study.
Introduction: Stains are a crucial component of laboratory procedure. They help in highlighting the different tissues, both normal and abnormal, which plays an important role in diagnostic histopathology. Synthetic dyes are utilised for majority of the stains in histology. Among the natural surrogates studied, Kumkum is less researched. Aim: To compare the staining characteristics of Kumkum, which imparts red colour as an alternative to eosin. Materials and Methods: This observational study was conducted at Histopathology section, Pathology department at Chettinad Hospital and Research Institute for a period of six months from January 2022 to June 2022. In 50 tissue blocks, each was made into two sections and one was stained with routine Haematoxylin and Eosin (H&E), while the other was stained with Haematoxylin and Kumkum solution (H&K). The Cellular Architecture (CA) (based on distinct or indistinct nucleus, cytoplasm) and quality of staining (poor, satisfactory or good) of H&E and H&K solution was compared and a scoring system was given. The scores were analysed with Chi-square test using SPSS software (version 25). Results: Out of 100 slides, 45 (90%) stained with H&E and 48 (96%) with H&K showed distinct CA. A total of 47 slides (94%) stained with H&K and 41 (82%) with H&E showed good quality of staining. Conclusion: Kumkum solution appears to be an efficient counterstain in place of eosin in highlighting the normal structures in histopathological sections. It highlighted the RBCs in the arteries and cytoplasm of the cells in glandular and squamous epithelium better than or equally good as eosin.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.