Pempek merupakan salah satu makanan tradisional ciri khas Palembang. Pempek sudah dikenal masyarakat secara luas. Masyarakat tidak terlalu susah untuk mendapatkan pempek di jajanan di sekitaran Garut Kota. Pembuatan pempek ini meggunakan bahan dasar utama daging ikan dan sagu. Beberapa temuan mengenai penggunaan boraks pada pempek di beberapa tempat. Penelitian bertujuan untuk mengidentifikasi dan menganalisis penggunaan borak pada empek yang dijual di sekitar kecamatan Garut Kota. Penelitian ini merupakan studi deskriptif dan laboratorium dengan tujuan untuk mengidentifikasi dan melakukan penetapan kadar boraks menggunakan analisis kualitatif dengan kurkumin dan analisis kuantitatif dengan titrasi asam basa. Sampel pempek yang peroleh dari 20 pedagang yang terseber di kecamatan Garut Kota. Berdasarkan hasil penelitian, secara kualitatif teridentifikasi 3 sampel pempek yang positif mengandung boraks. Hasil dari pemeriksaan organoleptis dari 20 sampel pempek, 3 sampel mempunyai tekstur yang sangat kenyal dan berbau seperti zat kimia sedangkan pada sampel pempek tidak mengandung boraks. Pengujian boraks dilanjutkan pada penetapan kadar atau analisa kuantitatif. Kandungan boraks pada ketiga sampel adalah 120 mg/g, 77,1 mg/g, dan 40,9 mg/g Dari hasil tersebut, disarankan agar masyarakat bisa membedakan dan memilih jajan pempek yang tidak mengandung boraks.
Metode pemeriksaan kreatinin dengan reaksi Jaffe memilki kekurangan dalam sensitivitas, reproduktifitas, dan presisi karena adanya senyawa yang dapat mengganggu pemeriksaan hingga menyebabkan over estimasi nilai kreatinin. Deproteinisasi serum sebelum reaksi merupakan pendekatan terbaik untuk menghilangkan semua bentuk gangguan, pada umumnya mengunakan penambahan TCA (Trikoloro asetat). Senyawa lain dapat digunakan dalam deproteinisasi dalam senyawa biologis darah, seperti asetonitril. Penelitian ini bertujuan untuk menguji berbedaan hasil pemeriksaan kreatinin dengan penggunanaan asetonitril dan TCA dalam proses deproteinisasi untuk menurunkan over estimasi kreatinin. Metode penelitian yang digunakan adalah penelitian komparatif untuk bisa mengungkapkan seberapa besar perbedaan hasil pengukuran kreatinin setelah dilakukan deprotenisasi dengan menggunakan asetonitril dan TCA sebagai standar pada serum yang berasal dari 20 orang pasien hemodialisa. Hasil penelitian menunjukan rata-rata pemeriksaan hasil kreatinin setelah deproteinisasi TCA lebih besar dibandingkan asetonitril dengan uji perbedaan mempelihatkan nilai signifikansi 0,016 (p<0,05). Hal ini menunukan bahwa penggunaan asetonitril menurunkan over estimasi nilai kreatinin lebih besar dibandingkan TCA secara signifikan.
Deparaffinization in the hematoxylin eosin staining process aims to remove paraffin from the tissue, clean the tissue and maximize dye absorption. Utilization xylol as deparaffinization has a drawback, if inhaled too much it will cause harm to the health of the laboratory staff. Considering this, a safer alternative to xylol is needed, one of which is dish washing soap. This study was conducted to determine the use of liquid dish soap at the deparaffinization stage on the quality of tissue staining results. The research method in this research is a literature review study conducted based on sources from several articles published digitally in the Science Direct, Pub Med, NCBI, Researchgate, Google Scholar with the keywords deparafinization, xylene, dish washing soap and Hematoxylin-eosin. Based on the results of a review of ten journals, the concentration of dishwashing soap used was 1.5%, 1.7%, 2% and 2.5% with the most widely used concentration of 1.7%. The most used deparaffinization time with dish soap was 1 minute, one study used 2 minutes. Of the several differences in deparaffinization time and concentration of dish soap, the results adequacy of nuclear and cytoplasmic staining, clarity, uniformity and crispness of staining were very good in almost all studies, there was no difference in quality with the use of xylol. So it can be concluded that the use of dish soap in the deparaffinization process for Hematoxylin staining showed good staining of all tissues. Dish soap can be used as an alternative agent in deparaffinization of Hematoxylin-Eosin staining.
Mannitol Salt Agar (MSA) is a selective and differential medium for isolating Stapylococcus bacteria, distinguishing Staphylococcus aureus bacteria from other species from the ability of mannitol to ferment which changes the color of the phenol red indicator from red to yellow. The use of synthetic indicators that are not environmentally friendly and difficult to decompose can be replaced with natural indicators, including the use of secang wood extract (Caesalpinia sappan L.) and purple cabbage (Brasicca oleraceae). The research was conducted using a descriptive method which describes the color changes of the modified mannitol salt agar (MSA) media of secang wood and purple cabbage extract after being planted with bacteria. Staphylococcus aureus and Coagulation Negative Staphylococcus (CoNS) bacteria were isolated from acne-prone skin. The growth of Staphylococus aureus changed the color of the modified MSA medium from red to yellow for the secang wood extract and the color change from blue to pink for the purple cabbage extract. The growth of CoNS bacteria changed the color of the modified MSA medium from red to red-orange for the secang wood extract and the color change from blue to blue-purple for the purple cabbage extract. Both bacteria thrived on purple cabbage modified MSA media. From this study, it was concluded that secang wood extract and purple cabbage could be used as natural indicators of modified MSA in growing Staphylococcus isolated from the skin.
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