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There is a limited genetic variability available in genetic resources of upland cotton (Gossypium hirsutum L.), which restricted the breeding activity. The objective of present study was evaluating the genetic variability in RIL population derived through intraspecific hybridization between RHAP 12 and RHAP 15 genotypes. The 222 RIL were evaluated during kharif 2018 at University of Agricultural Sciences Dharwad. The RILs were evaluated for agronomic and fibre quality traits such as plant height (cm), number of monopodial branches, number of sympodia branches, boll per plant, seed cotton yield per plant (g), seed Index (g), boll weight (g), lint index(g), ginning out turn (%), fiber strength (g/text), fiber length (mm), micronaire value (µg/inch), maturity coefficient (%), fiber elongation (%) and fiber uniformity ratio (%). The RIL were exhibited the large variation for seed cotton yield per plant i.e. PCV of 34.44 per cent and GCV of 28.05 per cent. Similarly number of bolls per plant also exhibited the high genetic variation with PCV and GCV of 28.26 and 21.34 per cent respectively. Even though after utilizing diverse parents in hybridization program observed the limited variability with respect to fibre quality traits. The high heritability and genetic advance over the mean registered in seed cotton yield per plant indicating that additive gene action. The desirable association with yield was found between number of bolls per plant, number of sympodia per plant, boll weight and maturity ratio. It is concluded that RIL population had high potential for enhancing the yield and fibre quality traits. K e y w o r d sCotton, Recombinant inbred lines (RIL), phenotypic (PCV) and genotypic (GCV) coefficient of variation

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