The study aimed to detect osteopontin (OPN) transcript in seminal plasma and to assess the relationship between the presence of the transcript and post-freeze-thaw quality of cryopreserved spermatozoa in mithun (Bos frontalis). Semen samples were collected from five adult bulls through rectal massage method and cryopreserved in tris-egg yolk-glycerol extender. OPN transcript was detected by RT-PCR in the RNA purified from seminal plasma. OPN transcript was found to be present consistently in three animals (OPN+) and absent in two animals (OPN-). Although, sperm viability and acrosomal integrity were found similar at different stages of cryopreservation in both the groups, tail abnormality after final dilution and, head and tail abnormalities after equilibration were found significantly (P less than 0.05) lesser in the OPN+ compared to OPN- animals. The results indicated that OPN is probably important in stabilizing sperm membrane that resulted in better resistance of sperm to cryopreservation process in OPN+ animals.
A study was conducted to investigate the various glycerol concentrations on freezability of mithun semen. The present study was conducted in semen production laboratory, ICAR-NRC on Mithun, Medziphema, Nagaland, India. A total of 20 ejaculates were collected from apparently healthy mithun bulls (n=6) by trans-rectal massage method. These ejaculates were extended with tris-egg yolk-citrate-glycerol diluent (TECG) and 5, 6 and 7% (v/v) glycerol, respectively were used as a cryoprotectant. Extended semen was equilibrated, cryopreserved and stored in liquid nitrogen with standard protocols. Semen quality parameters (SQPs) such as percentage of forward progressive motility, viability (Eosin Nigrosin staining), acrosomal integrity (Giemsa staining) and total morphological abnormality (Eosin Nigrosin staining) were estimated with standard procedure at different stage of preservation and with different concentration of the glycerol. The result revealed that these semen quality parameters were differed nonsignificantly among the three experimental groups at immediately after final dilution (Stage: 1) and differed significantly (p<0.05) at 4 hrs of equilibration (Stage: 2) and at post thawing stage (Stage: 3) of semen cryopreservation. Further, 5% glycerol treated group has shown significantly (p<0.05) higher values of motility and live with acrosomal integrity and lower value of total morphological abnormality than in 6% or 7% glycerol treated groups. Based on the result, it was concluded that 5% glycerol was best for the TECG diluent for preserving mithun semen in liquid nitrogen.
The present study was conducted to study the efficacy of different medicaments on postpartum anoestrus crossbred cows. Total of 20 post partum anoestrus crossbred cows were selected based on the case history and rectal palpation from the private dairy farm in and around the Khanapara, Guwahati, Assam. The animals were split into two groups (n=10) and treated with Vitamin A+Tonophosphan (Group 1), Vitamin A+Tonophosphan+Genital Massage (Group 2). Result of experiment revealed that number of animal responded and conceived were higher and duration between the initiation of treatment and expression of heat was shorter in group 2 treatment and expression of various heat signs were more prominent in this group 2 treatment. Similarly the biochemical profiles were higher in responded animal than non-responded and in group 2 treatment. Moreover, the biochemical profiles were significantly higher in day of induced oestrus, day 20 than day 10 or 0 of treatment in responded animal and higher in group 2 treatment. It was concluded that group 2 (Vitamin A+Tonophosphan+Genital Massage) treatment was given better response to the postpartum anoestrus in crossbred cows.
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