Efficacy of Heatsynch protocol for induction of estrus, synchronization of ovulation and timed artificial insemination in yaks (Poephagus grunniens L.)
“…Reproduction in yaks is low as a result of seasonal breeding, delayed puberty, and lower estrus frequency. Therefore, numerous recent studies have focused on reproduction and physiology in yaks, as well as assisted reproductive techniques (Sarkar et al, 2008; Guo et al, 2012). Follicular development is a very complicated process; it plays a role in oocyte maturation and the ovulation mechanism during yak breeding season.…”
Section: Discussionmentioning
confidence: 99%
“…Their natural habitat ranges from between 2,500 to 6,000 m above sea level. Yaks are seasonally polyestrous, exhibiting cyclic ovarian activity and estrus from July to November (Sarkar and Prakash, 2005; Sarkar et al, 2008). The length of the estrous cycle lasts from 19 to 21 days.…”
The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification. The expression level of transferrin and enolase superfamily member 1 (ENOSF1) was determined by Western blotting for verification analysis. The results showed that 2-DE obtained an electrophoresis map of proteins from mature and immature yak FF with high resolution and repeatability. A comparison of protein profiles identified 12 differently expressed proteins, out of which 10 of them were upregulated while 2 were downregulated. Western blotting showed that the expression of transferrin and ENOSF1 was enhanced with follicular development. Both the obtained protein profiles and the differently expressed proteins identified in this study provided experimental data related to follicular development during yak breeding seasons. This study also laid the foundation for understanding the microenvironment during oocyte development.
“…Reproduction in yaks is low as a result of seasonal breeding, delayed puberty, and lower estrus frequency. Therefore, numerous recent studies have focused on reproduction and physiology in yaks, as well as assisted reproductive techniques (Sarkar et al, 2008; Guo et al, 2012). Follicular development is a very complicated process; it plays a role in oocyte maturation and the ovulation mechanism during yak breeding season.…”
Section: Discussionmentioning
confidence: 99%
“…Their natural habitat ranges from between 2,500 to 6,000 m above sea level. Yaks are seasonally polyestrous, exhibiting cyclic ovarian activity and estrus from July to November (Sarkar and Prakash, 2005; Sarkar et al, 2008). The length of the estrous cycle lasts from 19 to 21 days.…”
The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification. The expression level of transferrin and enolase superfamily member 1 (ENOSF1) was determined by Western blotting for verification analysis. The results showed that 2-DE obtained an electrophoresis map of proteins from mature and immature yak FF with high resolution and repeatability. A comparison of protein profiles identified 12 differently expressed proteins, out of which 10 of them were upregulated while 2 were downregulated. Western blotting showed that the expression of transferrin and ENOSF1 was enhanced with follicular development. Both the obtained protein profiles and the differently expressed proteins identified in this study provided experimental data related to follicular development during yak breeding seasons. This study also laid the foundation for understanding the microenvironment during oocyte development.
“…We tested the efficacy of induction of oestrus, synchronization of ovulation and timed artificial insemination in anoestrous yaks using the Heatsynch protocol (Sarkar et al. 2007e).…”
Section: Endocrine Biotechniques To Enhance Fertilitymentioning
Yak and mithun are two domesticated herbivores of high economic importance to the farming community living in highlands. Improved yak and mithun production could significantly enhance the living standards of these highlanders. Over the years, their dwindling numbers have been a cause of serious concern. In view of the lack of knowledge on the reproductive physiology of these ruminants, studies have been undertaken to investigate their reproductive endocrinology in recent years. This paper attempts to present the latest information on the endocrine changes associated with their various reproductive processes viz. growth and puberty, oestrous cyclicity and oestrous behaviour, ovulation, and, pregnancy and parturition. The paper also provides the recent developments on research done towards the enhancement of yak and mithun reproductive efficiencies through endocrine and embryo biotechniques.
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