Detection of osteopontin transcript in seminal plasma and its association with post-freeze-thaw quality of cryopreserved spermatozoa in mithun (Bos frontalis)

Baruah, K. K.; Dhali, A.; Bora, B.; Mech, A.; Mondal, Mohan

doi:10.18805/ijar.v0iof.4553

Cited by 4 publications

(5 citation statements)

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“…Erikson (2006) conducted in vitro studies but could not establish any direct relationship between the OPN concentration and morphological aspects of sperms. Significant positive correlation between OPN level and the percentage of plasma membrane intact spermatozoa were in accordance with the findings of Baruah et al (2017) that documented the role of OPN in stabilizing the sperm plasma membrane and resist damages occurring during cryopreservation process. Highly significant positive correlation between OPN level and the percentage of acrosomal intact spermatozoa simulate the findings of Cancel et al (1999) who stated that the amount of OPN was more in acrosomal intact spermatozoa and vice versa.…”

Section: Antioxidant Enzymessupporting

confidence: 89%

Seminal Plasma Osteopontin: A Marker for Potential Fertility in Dogs

Abedin

Leela

Devendran

et al. 2020

IJAR

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Background: Osteopontin (OPN) is an extracellular matrix protein with multifunctions and has been identified in the reproductive tract of both males and females. OPN from the seminal plasma (SP) has been closely associated with male fertility in several domestic animals. Different isoforms of OPN has been identified in the SP and sperm membrane in dogs but with unknown functions. The current study investigated the comparison of key canine sperm microscopic parameters and SP markers between dogs exhibiting different fertility potential.Methods: In this investigation during 2018-2019, 21 dogs coming for breeding soundness examination were selected and divided into fertile (Group I) and hypofertile (Group II). Evaluation included assessment of sperm microscopic characteristics and estimation of OPN, Malondialdehyde (MDA), Catalase (CAT), Glutathione peroxidase (GPx) and Superoxide dismutase (SOD) concentration in the SP.Result: The OPN level showed positive correlation with all the sperm evaluated characteristics (p less than 0.01). Linear regression analysis depicted a significant (p less than 0.001) influence of OPN on the evaluated sperm characteristics. These findings suggest that OPN in the SP along with the microscopic characteristics of the spermatozoa can be used as a potential fertility marker in discriminating fertile from the hypofertile ones.

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Section: Antioxidant Enzymessupporting

confidence: 89%

Seminal Plasma Osteopontin: A Marker for Potential Fertility in Dogs

Abedin

Leela

Devendran

et al. 2020

IJAR

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“…Research on mithun has shown the essential role of OPN in membrane stability. It results in a better resistance of spermatozoa to cryopreservation [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…The osteopontin (OPN) gene is one of the genes studied. However, research on banteng spermatozoa is still limited and uses conventional approaches [ 20 , 21 ]. Osteopontin is synthesized by the ampullary accessory gland epithelial cells [ 22 ], and it is present in seminal plasma and is associated with the freezing ability of bovine semen [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Correlation between Post‐Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression

Yoelinda

Arifiantini

Solihin

et al. 2023

Veterinary Medicine International

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The role of ex situ conservation facilities or captivity through captive breeding programs is essential in the conservation of the endangered Javan banteng. The development of semen cryopreservation may assist on one side of the conservation plan. However, the male Javan banteng reproductive capability must be considered as it influences the targeted outputs. Studying the potential biomarker for fertility such as osteopontin gene expression is also expected to help predict male fertility. Therefore, this study aimed to analyze the quality of spermatozoa after thawing to help predict the male reproductive capability of Javan banteng. Furthermore, this study investigated the potential role of osteopontin gene expression in male Javan banteng fertility. A positive reinforcement approach was used to accustom the male and female animals as we focused on establishing a collection procedure using neither sedation nor anaesthesia. Semen samples were collected at Taman Safari Indonesia, Bogor, in accordance with the female banteng receptivity. Semen samples were then evaluated and then cryopreserved under field conditions. Our study showed the different predicted reproductive capability of the Javan banteng based on the post-thaw spermatozoa quality, which showed significant differences. The OPN gene showed positive correlations with the progressive motility (r = 0.711, p = 0.048), viability (r = 0.822, p = 0.012), and acrosomal integrity (r = 0.665, p = 0.072) of Javan banteng spermatozoa after thawing. Our study demonstrated the predicted Javan banteng reproductive capability based on various post-thaw spermatozoa variables. This finding is also the first report on the OPN gene potential to be developed as the assessment tool of post-thaw spermatozoa quality of the male Javan banteng. The findings in our study may help give recommendations for future breeding programs, especially in the ex situ conservation sites.

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“…Previous studies in stallions have shown positive effects of higher levels of OPN in seminal plasma on fertility. Additionally, research conducted by Baruah et al [29] highlighted the significant role of OPN in the cryopreservation process. In their study, OPN levels were positively correlated with various sperm characteristics.…”

Section: Comparative İnsights Of Opn On Fertility In Other Speciesmentioning

confidence: 99%

Osteopontin Concentration in Prostates Fractions: A Novel Marker of Sperm Quality in Dogs

Tekin,

Kurtdede,

Salmanoğlu

et al. 2023

Veterinary Sciences

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This study aimed to investigate the relationship between the sperm quality and the osteopontin (OPN) concentration in the prostates of Malakli shepherd dogs. Ejaculates were collected once by digital manipulation from 39 male dogs aged between 2 and 4 years and older. The first and third fractions of the ejaculate were centrifuged at 5000× g for 30 min, and supernatants were stored at −80 °C for further analysis of OPN using a double-antibody sandwich method (SEA899CA, Cloude-Clone Corp, Houston, TX, USA). Meanwhile, the second fractions were evaluated for sperm motility, concentration, viability, and rate of abnormal spermatozoa (head, acrosome, midpiece and tail abnormalities). The average concentration of OPN was 8.7 ± 5.2 ng/mL, and it differed significantly between the 1st 10.4 ± 5.3 ng/mL and 3rd 7.4 ± 5 ng/mL fractions. According to ROC (receiver operating characteristic curve) analysis, the OPN concentration had a better diagnostic ability for sperm motility (p < 0.001) than for the rate of abnormal spermatozoa (p < 0.05). Additionally, the OPN concentration was negatively correlated with poor sperm morphology and motility. In conclusion, the OPN concentration in prostate-derived secretions may be a possible marker of sperm quality in dogs. Further research could explore the involvement of OPN in sperm motility during cryopreservation and in vivo fertility.

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Detection of osteopontin transcript in seminal plasma and its association with post-freeze-thaw quality of cryopreserved spermatozoa in mithun (Bos frontalis)

Cited by 4 publications

References 0 publications

Seminal Plasma Osteopontin: A Marker for Potential Fertility in Dogs

Seminal Plasma Osteopontin: A Marker for Potential Fertility in Dogs

Correlation between Post‐Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression

Osteopontin Concentration in Prostates Fractions: A Novel Marker of Sperm Quality in Dogs

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