AbstrakHiperlipidemia merupakan salah satu penyebab penyakit jantung koroner yang ditandai dengan adanya penumpukan lemak yang disebabkan oleh kadar trigliserida tinggi. Hiperlipidemia dapat mengakibatkan perlemakan hati (fatty-liver) sehingga hepatosit mengalami kerusakan dan peningkatan aktivitas enzim trans-aminase. Hati memiliki peran penting untuk metabolisme lipid karena lipid diangkut di dalam darah sebagai lipoprotein yang dibentuk di dalam hati. Tujuan penelitian ini adalah untuk mempelajari pengaruh ekstrak biji ketumbar terhadap kadar trigliserida serum darah dan gambaran histologi hati tikus putih yang diinduksi pakan tinggi lemak. Penelitian menggunakan metode eksperimen, sampel sebanyak 24 ekor tikus putih galur Wistar dikelompokkan kedalam 6 kelompok, yaitu: K1 (tikus diberi pakan normal dan diberi akuades), K2 (tikus hanya diberi pakan tinggi lemak), K3 (tikus diberi pakan tinggi lemak dan simvastatin dosis 0,18 mg/kg BB/hari), K4, K5, dan K6 (tikus diberi pakan diet tinggi lemak dan diberikan ekstrak biji ketumbar dengan dosis masing-masing 5,4 mg/kg BB/hari, 10,8 mg/kg BB/hari, dan 21,6 mg/kg BB/hari). Hasil penelitian menunjukkan adanya penurunan kadar trigliserida serum darah tikus sebelum dan setelah diberikan ekstrak biji ketumbar. Pada gambaran histologi K1, K3, K4, K5, dan K6 didapatkan gambaran histologi hati yang normal, sedangkan pada K2 ditemukan sel kariolisis (nekrosis). Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak biji ketumbar berpengaruh terhadap penurunan kadar trigliserida serum darah tikus dan adanya perubahan histologi hati pada kelompok tikus yang diberikan ekstrak biji ketumbar dengan kelompok yang tidak diterapi.Kata kunci: biji ketumbar, trigliserida, histologi, hati, hiperlipidemia.AbstractHyperlipidemia is one of the causes of coronary heart disease marked by the accumulation of fat caused by high triglyceride levels. Hyperlipidemia can cause fatty liver so that hepatocytes experience damage and increase the activity of the transaminase enzyme. The liver has an important role in lipid metabolism because lipids are transported in the blood as lipoproteins formed in the liver. The purpose of this study is to investigate the effect of coriander seed extract on blood serum triglyceride levels and the histological picture of white rat liver induced by high-fat feed. The study used an experimental method in which a sample of 24 white rats were grouped into 6 groups, K1 (rats were given normal feed and given aqua dest), K2 (rats were only given high-fat feed), K3 (rats were given high-fat feed and simvastatin dose 0. 18 mg/kg/day), while K4, K5, and K6 (rats were fed a diet high in fat and given coriander seed extract at a dose of 5.4 mg/kg/day, 10.8 mg/kg/day, and 21.6 mg/kg/day respectively). The results showed a decrease in rat serum triglyceride level before and after coriander seed extract was given. In the histological features of K1, K3, K4, K5, and K6, a normal histological picture of the liver was found, while karyolysis cells (necrosis) were observed in K2 group. Based on the results of the study, the coriander seed extract might affect the reduction in rat serum triglyceride level as well as the histological changes of liver in rats treated with coriander seed extract as compared to the untreated group.Keywords: coriander seed, triglyceride, histology, liver, hyperlipidemia
Latar Belakang: Luka merupakan rusak atau hilangnya jaringan tubuh. Jika terjadi luka disekitar pembuluh darah yang rusak, maka akan terjadi proses penghentian pendarahan dan perbaikan jaringan sebagai respon tubuh melalui proses hemostasis. Babadotan (Ageratum conyzoides,L) merupakan salah satu tanaman yang memiliki kandungan senyawa aktif untuk mempercepat proses hemostasis.Tujuan Penelitian: Untuk mengetahui pengaruh pemberian ekstrak daun babadotan terhadap waktu pembekuan darah.Metode Penelitian: Metode penelitian yang digunakan yaitu quasy experiment (eksperimen semu). Rancangan ini berupaya untuk mengungkapkan hubungan sebab akibat dengan cara melibatkan kelompok kontrol dan kelompok eksperimen. Data yang diperoleh merupakan data primer yaitu data yang didapatkan dari hasil pemeriksaan waktu pembekuan darah dengan pemberian dan tanpa pemberian ekstrak daun babadotan dengan metode pemeriksaan Lee and White.Hasil: Rerata waktu pembekuan dari sampel darah yang tanpa perasan daun babadotan yaitu 9,89 menit, sedangkan sampel darah yang diberikan perasan daun babadotan menunjukkan waktu pembekuan 9,19 menit. Sehingga selisih waktu pembekuan pada kedua sampel adalah 0,7 menit atau lebih cepat sebesar 7%.Simpulan: Ekstrak daun babadotan (Ageratum lonyzoides,L) dapat digunakan untuk mempercepat waktu pembekuan darah.
The Severe Acute Respiratory Syndrome Coronavirus-2, or COVID, was announced by WHO on February 11, 2020. Examination of SARS-CoV2 by RTq-PCR method in Labkesda Kab. Sukabumi is one of the health service support units in the Sukabumi area. The number of PCR examination requests is increasing every day, whereas, at this stage, the temperature increase process should reach the recommended temperature of 90°C, which can take 20-25 minutes. Sometimes ATLM has processed specimens at 60°C - 90°C. Given the required temperature at the time of extraction, which is at a temperature of 90 degrees, here the researchers wanted to prove whether below 90 degrees had an effect on the virus extraction process. This study aims to determine the effect of differences in extraction temperature on the SARS-CoV2 RTq-PCR examination process on CT VALUE on patient specimens. The variable in this study was the difference in temperature consisting of 60°C, 80°C, and 90°C. This research used quantitative research methods with case studies. This study is an experimental study to determine the effect of temperature differences on the CT value of the RTq - PCR examination. The population of this study were 30 patients at LABKESDA Kabupaten Sukabumi. This analysis used a one-way ANOVA test analysis with software. IBM SPSS 23.0. It may be concluded that there is no significant difference in the CT Value findings on the SARS-CoV2 RTq-PCR Examination since the study's p-value was 0.758, which is more than 0.05.
The method of measuring Hb levels recommended by ICSH uses the HiCN method. However, it has a high risk due to the toxicity of the reagent. Therefore, the determination of Hb has been developed using the SLS method. According to Oshiro and Mansoor, who both stated that there was no significant difference between SLS and HiCN, this method has two distinct procedures. This study aims to determine the comparison between the measurement results of hemoglobin levels using sodium lauryl sulfate with the Oshiro procedure and Mansoor. The research design used is analytical research. The data obtained from the results of measuring Hb levels with the Oshiro and Manshoor procedures was given to students of the Diploma IV Study Program of Medical Laboratory Technology, Rajawali Health Institute batch 2018, which collected 49 people. The sampling technique used was saturated sampling to reduce the error rate in the study. The results of the normality test of the data in this study showed n of the two procedures was 49 mean±SD for the Oshiro procedure 13.09±0.56 and p = 0.200. Meanwhile, the Mansoor procedure had a meanSD of 13.09±0.57 and a p-value of 0.059. Because the probability of both being p > 0.05, the data is declared normally distributed. The results of the average difference test from the data of this study show the mean±SD of the pairwise difference between the Oshiro and Mansoor procedures is 0.002±0.059 and the p value = 0.811. If P > 0.05, then it is stated that there is no significant difference between the two groups. The conclusion of this study is that the result of measuring Hb levels using the SLS procedure is reliable. Oshiro and Mansoor conform to HiCN and there is no significant difference in the mean results.
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