Quantitative trait loci (QTLs) for yield and drought-related traits were exchanged via marker-assisted selection between elite cultivars of two cotton species, Gossypium barbadense (GB) cv. F-177 and Gossypium hirsutum (GH) cv. Siv'on. Three of the resultant near-isogenic lines (NILs), each introgressed with a different QTL region, expressed an advantage in osmotic adjustment (OA) and other drought-related traits relative to their recipient parents. These NILs and the parental genotypes were field-grown under well-watered and water-limited conditions, and characterized for their metabolic and mineral compositions. Comparisons were then made between (1) GB and GH genotypes, (2) the contrasting water regimes and (3) each NIL and its recipient parent. Hierarchical clustering analysis clearly distinguished between GB and GH genotypes based on either metabolite or mineral composition. Comparisons between well-watered and water-limited conditions in each of the genotypes showed differing trends in the various solutes. The greater concentrations of potassium, magnesium and calcium under water stress, when compared with well-watered conditions, may have enhanced OA or osmoprotection. All NILs exhibited significantly modified solute composition relative to their recipient parents. In particular, increased levels of alanine, aspartic acid, citric acid, malic acid, glycerol, myoinositol, threonic acid, potassium, magnesium and calcium were found under drought conditions in one or more of the NILs relative to their recipient parents. The increased values of these solutes could contribute to the superior capacity of these NILs to cope with drought.
The cause for the differences in germination ability of large and small confection sunflower (Helianthus annuus L.) seeds was investigated over 3 years. The source-sink relationship was manipulated to better explore the differences between seeds of various sizes and to study the role of the embryo and the pericarp (hull) in controlling germination ability. Percent germination of large seeds was significantly lower than that of small seeds when tests were performed at 15 °C. Increasing the ratio of leaf area to number of developing seeds caused an increase in mean seed mass, but resulted in a lower percentage of germination. Seed vigor, as measured by mean time to germination or to emergence of hulled seeds or by rate of root elongation, was negatively correlated with embryo mass, indicating that the low vigor of large seeds is not due to the mechanical barrier imposed by the hull. Analysis of electrolyte leakage confirmed the hypothesis that the low quality of large seeds results from a disturbance during the process of seed development.
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