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Raising chinchilla (Chinchilla laniger) for commercial purpose has increased significantly; however, hematological and serum biochemical reference values have not yet been determined for chinchillas raised in south Brazil. Establishing blood cells and serum biochemistry reference values might be helpful to evaluate health status of chinchillas and might be used as a tool by clinicians. The purpose of this study was to determine the reference values for blood cells and serum biochemistry of Chinchilla laniger. Blood samples were collected by cardiac puncture from 16 adult males, at the time they were killed to remove the fur coat, and from 8 adult males anesthetized with ketamine and xylazine. Blood cell counts and serum biochemistry analysis were performed using standard techniques and the results were expressed as mean ± SEM. Analysis of blood parameters from post-mortem cardiac punctured and from anesthetized chinchillas indicated that blood samples from anesthetized chinchillas had higher PCV, Hemoglobin, MCHC and WBC (P < .05); in contrast, had lower levels of monocytes, basophils and eosinophils (P < .05). Serum biochemical parameters were less affected by sampling method: anesthetized chinchillas had lower levels of urea, glucose and triglycerids (P < .05). The data obtained might be useful as a parameter to monitor the health status of chinchillas raised in south Brazil.

The stress in dorado fingerlings (Salminus brasiliensis) caused by transportation at densities of 5, 10, and 15g/l after 4, 8, and 12h was evaluated by the concentration of tissue cortisol measured by ELISA. The conditions of transportation were simulated on an orbital table shaker with horizontal movements, inside 15 litres plastic bags filled with 4 litres of water and pure oxygen. Cortisol concentrations increased in all densities after 4h of transportation converging to a common concentration at the end of the tested times. Electrical conductivity of water increased with density and transportation time. The transportation caused stress on fish, but the increase on density and in times of transportation did not cause mortality to fingerlings. The transportation of S. brasiliensis fingerlings can be done without mortality or apparent injuries to animals until the maximum analyzed density of 15g/l and up to 12h.

Intervalos de tempo entre enema e colonoscopia rígida no preparo intestinal com solução monobásica e dibásica de sódio em quatro cãesIntervals of the time of enema after rigid colonoscopy in the colon preparation with sodium phosphate monobasic an dibasic solution in four dogs RESUMOA colonoscopia é importante procedimento diagnóstico e terapêutico utilizado nas afecções que acometem o intestino grosso de cães. Uma condição essencial para sua realização é o preparo do paciente, incluindo a remoção de fluídos e do material fecal do cólon. Nesse estudo foram avaliados a eficácia do preparo com bisacodil oral e enema fosfatado, comparando dois diferentes intervalos de tempo entre o enema e a colonoscopia. Quatro cães foram distribuídos igualmente em dois grupos: grupo 2 horas (G2h) e grupo 45 min. (G45min.), de acordo com o intervalo de tempo transcorido entre o segundo enema e a colonoscopia. Em ambos os grupos, o colônica grau II foi classificado como bom, enquanto que o G45min. em grau I, excelente. A solução de NaP retal, promove limpeza colônica adequada para colonoscopia, porém, para melhor visibilização da mucosa, indica-se que o segundo enema seja realizado 45minutos previamente ao procedimento. ABSTRACTThe colonoscopy is a diagnostic and terapeutic procedure used in the dog's bowel. Patient preparation is an excencial aspect to this procedure, wich include removal of fluid and fecal material from the colon. On this report we evaluated the efficacy of oral bisacodil combined with a phosphate enema, were compared two differents interval of time, considering enema and colonoscopy. Four dogs separeted in two groups: group 2 hour (G2h) and group 45 min. (G45min.), according to the time interval between second enema and colonoscopy. In both groups the first enema was performed four hours before procedure. The G2h resulted in cleaning colonic grade II, classified as good visibilities, while G45min., showed grade I, excellent. The rectal application of NaP, promotes colonic cleaning suitable for colonoscopy. However, to better mucosal visualization, the second enema should be performed 45 minutes before colonoscopy.