BackgroundPropolis is the bee product noted for multiple biological effects, and therefore it is widely used for the prevention and treatment of a variety of diseases. The active substances of propolis are easily soluble in ethanol. However ethanolic extracts cannot be used in treatment of certain diseases encountered in ophthalmology, pediatrics, etc. Unfortunately, the main biologically active substances of propolis are scarcely soluble in water, oil and other solvents usually used in pharmaceutical industry. The aim of this study was to investigate chemical composition, radical scavenging and antimicrobial activity of propolis extracts differently made in nonethanolic solvents.MethodsTotal content of phenolic compounds in extracts was determined using Folin-Ciocalteu method. Chemical composition and radical scavenging activity of extracts were determined using HPLC system with free radical reaction detector. Antimicrobial activity of examined preparations was evaluated using the agar-well diffusion assay.ResultsTotal amount of phenolic compounds in extracts made in polyethylene glycol 400 (PEG) and water mixture or in PEG, olive oil and water mixture at 70 °C was comparable to that of ethanolic extract. Predominantly identified compounds were phenolic acids, which contribute ca. 40 % of total radical scavenging activity.Investigated nonethanolic extracts inhibited the growth and reproduction of all tested microrganisms. Antimicrobial activity of some extracts was equal or exceeded the antimicrobial effect of ethanolic extract. Extracts made in pure water or oil only at room temperature, contained more than 5 – 10-fold lower amount of phenolic compounds, and demonstrated no antimicrobial activity.ConclusionsNonethanolic solvent complex and the effect of higher temperature allows more effective extraction of active compounds from propolis. Concentration of total phenolic compounds in these extracts does not differ significantly from the concentration found in ethanolic extract. Propolis nonethanolic extracts have radical scavenging and antimicrobial activity.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-015-0677-5) contains supplementary material, which is available to authorized users.
The scientific interest for the search of natural means of microbial inhibitors has not faded for several years. A search of natural antibiotics, so-called bacteriocins which are produced by lactic acid bacteria (LAB), gains a huge attention of the scientists in the last century, in order to reduce the usage of synthetic food additives. Pure bacteriocins with wide spectra of antibacterial activity are promising among the natural biopreservatives. The usage of bacteriocin(s) producing LAB as starter culture for the fermentation of some food products, in order to increase their shelf-life, when synthetic preservatives are not allowable, is also possible. There are a lot of studies focusing on the isolation of new bacteriocins from traditional fermented food, dairy products and other foods or sometimes even from unusual non-food matrices. Bacteriocins producing bacteria have been isolated from different sources with the different antibacterial activity against food-borne microorganisms. This review covers the classification of bacteriocins, diversity of sources of bacteriocin(s) producing LAB, antibacterial spectra of isolated bacteriocins and analytical methods for the bacteriocin purification and analysis within the last 15 years.
Background
Avian infectious bronchitis (IB) is a disease that can result in huge economic losses in the poultry industry. The high level of mutations of the IB virus (IBV) leads to the emergence of new serotypes and genotypes, and limits the efficacy of routine prevention. Medicinal plants, or substances derived from them, are being tested as options in the prevention of infectious diseases such as IB in many countries.
The objective of this study was to investigate extracts of 15 selected medicinal plants for anti-IBV activity.
Results
Extracts of
S. montana
,
O. vulgare
,
M. piperita
,
M. officinalis
,
T. vulgaris
,
H. officinalis
,
S. officinalis
and
D. canadense
showed anti-IBV activity prior to and during infection, while
S. montana
showed activity prior to and after infection.
M. piperita
,
O. vulgare
and
T. vulgaris
extracts had > 60 SI. In further studies no virus plaques (plaque reduction rate 100%) or cytopathogenic effect (decrease of TCID
50
from 2.0 to 5.0 log
10
) were detected after IBV treatment with extracts of
M. piperita
,
D. canadense
and
T. vulgaris
at concentrations of extracts ≥0.25 cytotoxic concentration (CC
50
) (
P
< 0.05). Both PFU number and TCID
50
increased after the use of
M. piperita
,
D. canadense
,
T. vulgaris
and
M. officinalis
extracts, the concentrations of which were 0.125 CC
50
and 0.25 CC
50
(
P
< 0.05). Real-time PCR detected IBV RNA after treatment with all plant extracts using concentrations of 1:2 CC
50
, 1:4 CC
50
and 1:8 CC
50
. Delta cycle threshold (Ct) values decreased significantly comparing Ct values of 1:2 CC
50
and 1:8 CC
50
dilutions (
P
< 0.05).
Conclusions
Many extracts of plants acted against IBV prior to and during infection, but the most effective were those of
M. piperita
,
T. vulgaris
and
D. canadense
.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.