Chrysophyllum perpulchrum (Sapotaceae) is used in the traditional Ivory Coast pharmacopeia to cure fevers. The extract of C. perpulchrum used for this study was the powdered form obtained from the maceration of the dried plant bark in 96% methanol, followed by evaporation to dryness. In the present study, the antioxidative and radical-scavenging activities of the methanolic extract were studied with three standard biological tests: DPPH reduction, ferric thiocyanate (FTC) lipidic peroxidation inhibition and thiobarbituric acid reacting substances (TBARS). Gallic acid and quercetin were used as references. The total amount of phenolic compounds in the extract was determined by ultraviolet (UV) spectrometry and calculated as gallic acid equivalents. Catechin and two dimeric procyanidins were found to be the compounds responsible for the activities. They were chemically dereplicated in the extract by LC-MS. For quantitation purposes, they were isolated by successive chromatographic methods and characterized by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectrometry. The quantities of these compounds in C. perpulchrum were 5.4% for catechin (P1), and 5.6 and 9.2% for dimers (compounds 2 (P2) and 3 (P3)), respectively. They displayed antioxidant activity with IC50 values of 2.50 ± 0.15 µg/mL (P1), 2.10 ± 0.2 µg/mL (P2) and 2.10 ± 0.1 µg/mL (P3). The total extract, the active fractions and the pure compounds inhibited the lipid peroxidation by the FTC method and the TBARS method in the range of 60%. These values were comparable to those seen for quercetin.
Turraea heterophylla Smith (Meliaceae) is a species used in Ivorian's traditional medicine for its various properties, such as antimalarial and aphrodisiac. This study aims to determine the chemical composition of the leaves from T. heterophylla and their content in polyphenols, in addition the antibacterial activity of the methanolic extract. Chemical test and spectrophotometry methods were used for phyotchemical study. The antibacterial activity was assessed using the agar well diffusion method against Staphylococcus aureus (ATCC 25923), Escherichia coli (CIP 54127AF) and Pseudomonas aeruginosa (CIP 103467). Phytochemical study revealed the presence of polyterpenes, steroids, alkaloids, saponins, polyphenols and flavonoids. A very high content of flavonoids and polyphenols were observed in the ethyl acetate and aqueous extracts. The antibacterial tests indicated that the methanol extract of the leaves from T. heterophylla had bacteriostatic properties against the germs tested with MIC values greater than 3000 µg / mL.
Two sterols namely β-sitosterol (1) and stigmasterol (2) were isolated from methanolic extract of the fruit barks of Coelocaryon klainei Pierre ex Heckel (Myristicaceae). They were isolated for the first time from this species. It is the first isolation of compounds in this genus Coelocaryon. The structures were elucidated on the basis of one and two-dimensional NMR, SM, IR and UV.Akoubet et al., International Current Pharmaceutical Journal, August 2017, 6(9): 49-52http://www.icpjonline.com/documents/Vol6Issue9/01.pdf
Four compounds were isolated from the leaves of Monodora brevipes Benth. (Annonaceae). Among them, one new natural indole named 5-formylindole (1) and three known aporphine alkaloids: (+)-roemeroline (2); (+)-corydine (3) and (+)-menispermine (4). They were isolated for the first time from this species. The structures of these compounds were established according to their spectral data (NMR, SM, IR and UV).Eric et al., International Current Pharmaceutical Journal, June 2017, 6(7): 40-43http://www.icpjonline.com/documents/Vol6Issue7/01.pdf
Extracts of the leaves, stem, roots, and seeds of <em>Mitracarpus scaber</em> plants have been thoroughly investigated in an attempt to determine their phytochemical and antioxidant activities.<em> </em>Phytochemical screening carried out by thin-layer chromatography (TLC) revealed the presence of several secondary metabolites in all the selective extracts of the plant. The levels of phenolic compounds showed that the leaves of <em>M. Scaber</em> are the richest in polyphenols with an average value of 488.291 ± 1.205 μg GAE/g (microgram of gallic acid equivalent per gram of the dry matter of the powdered plant). The total flavonoid assay revealed a high content in the leaves (1.624 ± 0.032 %). Also, studies of the antioxidant activities by DPPH<sup>● </sup>(2,2-diphenyl-1'-picrylhydrazyl) method showed a significant effect compared to vitamin C (96.62 %). The percentages of inhibition are respectively 89.82 % and 82.3 % for ethyl acetate extracts of the leaves and seeds. Furthermore, the inhibitory percentages of n-butanol in the leaves and seeds are 90.57 % and 83.67 %, respectively. Calculated IC<sub>50</sub> showed that the n-butanol fraction of the seeds exhibited the highest activity with an IC<sub>50</sub> of 0.293 mg/mL less than ascorbic acid with an IC<sub>50</sub> of 0.387 mg/mL.
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