This study evaluated the effects of comorbid disorders of diabetes and hyperthyroidism in the adult male mice. In total, 32 ICR strain mice were equally distributed into four groups: control (C), diabetic (D), diabetic-plushyperthyroid (DH), and hyperthyroid (H). Mice allocated for diabetes received a single intraperitoneal injection of streptozotocin (STZ) at 200 mg/kg body weight. At the onset of diabetes, one group of mice was concomitantly injected levothyroxine (LT4; 0.3 mg/kg body weight) and the other set of animals received the same treatment independently on a daily basis. The body weight, as well as the testicular and epididymal weights, was reduced markedly in D and DH mice. Higher trends of blood glucose levels were seen in the DH group, in comparison to euthyroid diabetic mice. Thyroid hormones could exert a transient effect on blood glucose homeostasis by altering the serum blood glucose level in diabetic patients. Histomorphometric analysis showed increased luminal sizes of seminiferous tubules, along with decreased epithelial height and atrophic changes in germinal stem cells in the testis of DH and H mice. Caput epididymis of DH mice showed extensive compaction of principal cells, loss of stereocilia, lipid vacuolization, and inflammatory infiltrations; however, damaged tubular integrity, packed clear cells, exfoliated cells, and round spermatids were profoundly noticed in the cauda epididymis. Hyperthyroidism elevated the serum testosterone levels in H and DH mice and produced critical damages to the histoarchitecture of the epididymis. Collectively, this experiment endeavored to mimic the polyglandular autoimmune syndrome, which will be helpful to better understand the reasons for male infertility in diabetic-cum-hyperthyroid patients.

BackgroundDiabetes and hypothyroidism produce adverse effects on body weight and sexual maturity by inhibiting body growth and metabolism. The occurrence of diabetes is always accompanied with thyroid dysfunction. Thus, it is important to take hypo- or hyper-thyroidism into consideration when exploring the adverse effects caused by diabetes. Previous reports have found hypothyroidism inhibits testicular growth by delaying Sertoli cell differentiation and proliferation. Hence, by establishing a mouse model of diabetes combined with hypothyroidism, we provided evidence that poly glandular autoimmune syndrome affected testicular development and spermatogenesis.Resultswe mimicked polyglandular deficiency syndrome in both immature and prepubertal mice by induction of diabetes and hypothyroidism, which caused decreases in serum concentrations of testosterone and insulin like growth factor 1 (IGF-1). Such reduction of growth factor resulted in inhibition of testicular and epididymal development. Moreover, expressions of Claudin-11 were observed between Sertoli cells and disrupted in the testes of syndrome group mice. We also found reduced sperm count and motility in prepubertal mice.ConclusionsThis mimicry of the diabetes and thyroid dysfunction, will be helpful to better understand the reasons for male infertility in diabetic-cum-hypothyroid patients.Electronic supplementary materialThe online version of this article (10.1186/s12861-018-0174-4) contains supplementary material, which is available to authorized users.

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T he antibiotic development promoters as feed additives have been forbidden in many countries due to cross-resistance adjacent to pathogens and residues in tissues of animal and poultry (Hajati et al., 2014). There are several evidences have been reported by different scientists on influence of antimicrobial drugs on the unlike herbal extorts in various parts of world (Rajput et al., 2012;Jarić et al., 2015;Alabi et al., 2017). Medicinal herbs and their extracts are among the alternative sources of allopathic growth enhancers are already being in use ( Jarić et al., 2015). The medicinal herbs could be an excellent alternative source of allopathic growth promoters, with lower expenditure of manufacturing, and abridged danger of toxicity, least hazards for health and friendly environment, since the physical condition of intestinal gut plays vital role in the continuance of better development with most important function of absorption Abstract | The present study was designed to evaluate the effect of herbs on the performance of broiler chickens challenged with coccidiosis. A total of 450 broiler chickens were allotted into 6 groups (n = 75/group). Group A controlled without supplementation, Group B, C and D were supplemented with Aloe barbadensis (5ml/L), Ferulafoetida regal (500mg/L), Tamarindus indica (50mg/L), respectively. Group E and F were supplemented with a mixture of all 3 herbs/plants at 2ml/L; mixed with distilled water and another with citric acid. The dressing percentage was observed significantly (P < 0.05) higher in compound II supplementary group F and lowest in control group A. Whereas, the fat pad percentage recorded significantly (P < 0.05) lowest in the compound II supplementary group F and highest in control group A. The relative weight of organs; liver, pancreas, spleen, heart, duodenum, jejunum, ileum, thymus and ceaca was observed significant (P < 0.05) in the herbs supplementary groups as compared to control group. The compound-II supplementary group F significantly (P < 0.05) increased the percentage of crude protein and fat contents as compared to control and other groups. Histomorphological examination of liver of broiler chicken supplemented with Compound-I and Compound-II in the coccidiosis challenged broiler chickens showed few pathological changes as compared to control (coccidiosis induced and without supplementation) group. In conclusion, supplementation of herbs mixture (Group E and F) at the dose 2ml/L to the coccidiosis challenged broiler chickens improve the overall performance and liver function.

Purpose The purpose of this paper is to explore halal meat production potential and export potential in Pakistan. Following this, the paper is to both stimulate the business and encourage research within this field. Design/methodology/approach For this study, both primary and secondary data were used. Time-series meat production and export data from 1994-1995 to 2015-2016 were collected from different secondary sources; where primary data were collected from distinguish livestock experts on the possibility of halal meat production in various provinces of the country. Findings Results show that during 1994-1995 to 2015-2016, annual average growth rates of meat production and export were 3 and 32%, respectively. Based on these growth rates, it was estimated that Pakistan's total meat production and export is projected to be 6,078 thousand tons and $17,477m by 2029-2030. Results regarding the expert opinion survey indicates that Balochistan province has comparative advantage to rare livestock for beef and mutton meat production over rest of the provinces; there is potency for producing chicken meat in Khyber Pakhtunkhwa (KPK) and Punjab provinces, while Sindh province has almost the same efficiency to produce beef and chicken meat. Practical implications Experts were in opinion that high yielding livestock breeds should be introduced to farmers; and comprehensive training programs should be designed for all the stockholders involved in meat production, and processing to exports chains. Therefore, it is suggested that registered and corporate livestock farming can be only solution to fulfill required standards of raw and processed meat business in international markets. Originality/value At current, halal meat export is considered as major activity of livestock sector of Pakistan: where, this is one of the pioneer studies exploring potential of halal meat production in various parts of the country, which can not only contribute in national income but support the balance of payments of the country.

This research was aimed to determine the impact of milk source on mozzarella cheese's chemical, rheological, and organoleptic characteristics. A total of 10 lots of cow and buffalo milk mozzarella cheese each were prepared. Prominent milk source influence on the chemical characteristics and calorific values of mozzarella cheese was determined. A decrease in moisture content and an increase in fat, protein, ash, and calorific values were noted in mozzarella cheese against their respective milk. Moisture content in cow milk mozzarella cheese (CMM) was considerably higher than buffalo milk mozzarella cheese (BMM). Average fat, ash content and calorific values appeared considerably high in BMM cheese compared to CMM cheese but protein content was recorded inverse and statistically non-significant (P>0.05). The average meltability was slightly higher in CMM cheese and the stretchiness of CMM cheese appeared significantly high from that of BMM cheese. BMM cheese was remarkably high in yield in contrast to that of CMM cheese.Appearance, flavor, and body/texture scores were slightly higher in CMM cheese as compared to BMM cheese. Regardless, the average benefit computed for BMM cheese was high in comparison to CMM cheese, the differences among them were statistically nonsignificant. The present study concludes that buffalo milk is better to produce mozzarella cheese with higher calorific values, better yield, and with more economic values, while cow milk is better to produce mozzarella cheese with the more proteinous, enhanced stretchiness, and better organoleptic quality.

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