Contemporaneous effects of diabetes mellitus and hypothyroidism on spermatogenesis and immunolocalization of Claudin-11 inside the seminiferous tubules of mice

Korejo, Nazar Ali; Wei, Quanwei; Zheng, Kaizhi; Mao, Dagan; Korejo, Rashid Ali; Shah, Atta Hussain

doi:10.1186/s12861-018-0174-4

Cited by 7 publications

(8 citation statements)

References 53 publications

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“…One explanation could be that QIg extract possess a strong antioxidant activity that play a vital role in chelating metal ions, scavenging intracellular free radicals and anti-inflammatory abilities (44). In addition, the current study indicates that combined diabetes and hypothyroidism can stop rat spermatogenesis, these results were consistence with (29). Treatment of diabetic rats with QIg extracts improved testicular dysfunction by restoration of seminiferous tubule deformities and may be simulation of activity of spermatogenic cells.…”

Section: Discussionsupporting

confidence: 55%

“…However, reduced serum testosterone level did not recover in treated diabetic rats after 15 days of treatment with different doses of QIg extract. One possible explanation could be the contemporaneous influence of both the diabetes and hypothyroidism on the reduction of serum testosterone concentrations (29), which might need longer duration of treatment with QIg extract to restore testicular function. However, neither diabetes nor QIg extract treatment had any effect on the LH secretion could be explained that the activity of pituitary gland was not changed by day 15 of streptozotocin-induced diabetes mellitus in rats (40).…”

Section: Discussionmentioning

confidence: 99%

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Impact of Quercus infectoria Galls Extract on Thyroid Gland and Testicular Functions in Diabetic Rats

Ibrahim

2021

Iraqi J. Vet. Med.

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Diabetes mellitus has been reported to be accompanied by thyroid and testicular dysfunctions. The objective of this study was to investigate the effect of Quercus infectoria galls (QIg) extract on the thyroid gland and testicular functions in diabetic rats. Sixteen rats were randomly divided into four equal groups, consisting of normal control, diabetic untreated control, diabetic treated with oral administration of 500 mg/kg BW and 1000 mg/kg BW, respectively for 15 days. Serum blood glucose, thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), testosterone (T), and luteinizing hormone (LH) were assessed. At the end of the experimental period, the rats were euthanized for histopathological analysis of thyroid gland and testis. Furthermore, immunohistochemistry was used to assess the expression of thyroid transcription factor-1 (TTF-1) in the thyroid gland of rats. The significant increase in serum blood glucose level in diabetic rats (DC) was markedly decreased by treatment with QIg extract (500 mg and 100 mg/kg BW) almost to the normal level. The reduced thyroid hormones, both the T3 and T4 were significantly recovered after 15 days of treatment with QIg extract (500 mg and 100 mg/kg BW). Whereas serum concentration of testosterone was significantly reduced in diabetic rats with QIg extract (500 mg and 100 mg/kg BW) treatment. Histopathological analysis of diabetic rats showed a wide range of morphological alterations in thyroid gland and testicular structures, which were almost completely, restored back to normal by treatment of rats with QIg extract. Furthermore, results showed overexpression of TTF-1 in the thyroid gland of diabetic rats, which was recovered back to normal expression after 15 days of treatment with QIg extract. These findings may provide new insights into the potential role of QIg extract as a promising therapeutic agent against diabetic complications in thyroid gland and testicular functions.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Impact of Quercus infectoria Galls Extract on Thyroid Gland and Testicular Functions in Diabetic Rats

Ibrahim

2021

Iraqi J. Vet. Med.

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“…The measurement of DAB color intensity using the image pixels was performed according to a previously published method [35] and in accordance with our previous laboratory study [36]. Briefly, the DAB-and hematoxylin-stained IHC digital images captured at 400× magnification were used for analysis through ImageJ software that contained a compatible plug-in of the IHC profiler.…”

Section: Methodsmentioning

confidence: 99%

Resveratrol Protects against Restraint Stress Effects on Stomach and Spleen in Adult Male Mice

Ennab

Mustafa

Wei

et al. 2019

Animals

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Simple SummaryThe current project was designed to examine the effects of restraint stress on body weights, food and water consumption, and pathophysiology of the stomach and spleen in adult mice, and potential roles of the phenolic compound resveratrol during treatment. We found that restraint stress (which is known to be a mixture of psychologic and physical stress) caused a reduction in body weight, food and water consumption, and morphologic changes in the stomach and spleen, which could lead to gastritis or ulcers and splenomegaly, while treatment with resveratrol prevented the negative alterations to the stomach and spleen.AbstractThe objectives were to investigate whether restraint stress (which is known as a mixture of psychologic and physical stress) exerts negative effects on the stomach and spleen, and whether the phenolic compound resveratrol (RES) exerts any protective roles. Fifty adult male mice were divided into five groups, with 10 mice per group as follows: control (C), restraint stress (RS), RS with vehicle (RS + V), RS with 2 mg/kg of resveratrol (RS + 2 mg RES), and RS with 20 mg/kg of resveratrol (RS + 20 mg RES). Mice were restrained in conical centrifuge tubes for 4 h daily to establish the RS model. RS + 2 mg RES, RS + 20 mg RES, and RS + V groups were given an oral dose of resveratrol or vehicle for 15 consecutive days, while the control group was not exposed to restraint stress. Herein, we showed that restraint stress decreased body weight and food and water consumption in stressed groups RS and RS + V compared to controls, while the groups treated with resveratrol showed improvements. Moreover, restraint stress caused acute damage to the morphology of gastric cells and reduced the quantitative distribution of parietal cells along with their decreased size and diameter, pointing to gastritis or ulcer. Furthermore, the antibody against the apoptosis-inducing factor (AIF) was highly attached in the RS groups. Splenic size, weight, and length were also greatly augmented in the stressed groups compared to the controls, while these phenomena were not observed in the RS + 2 mg RES group. Our findings proved significant ameliorating effects of resveratrol against restraint stress in adult male mice.

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“…The overproduction of ROS overcharges the natural antioxidant system, leading to LP, cellular, and organelle membrane damage, thus contributing to DM complications 15,65 . In addition, testicular damage is accompanied by epididymal injury; serious alterations in epididymal architecture were already found in rats under induced‐DM due to lipid peroxidation 66–68 . The histological modifications of the epidydimal tissue suggest disequilibrium in its microenvironment, which is necessary for adequate sperm maturation.…”

Section: Discussionmentioning

confidence: 99%

“…15,65 In addition, testicular damage is accompanied by epididymal injury; serious alterations in epididymal architecture were already found in rats under induced-DM due to lipid peroxidation. [66][67][68] The histological modifications of the epidydimal tissue suggest disequilibrium in its microenvironment, which is necessary for adequate sperm maturation.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol improves sperm DNA quality and reproductive capacity in type 1 diabetes

et al. 2020

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Background In the coming decades, diabetes mellitus might affect 628 million individuals. Its final impact on male fertility and reproductive outcomes should be considered since the number of adolescents and young adults presenting diabetes is rising. Resveratrol (RES), a polyphenol, is a biological modulator with multitarget and multi‐action characteristics. Objectives to evaluate if RES is effective against the male reproductive damage caused by type 1 diabetes (DM1), focusing on sperm DNA integrity and reproductive outcome. Materials and methods At 30 dpp (days postpartum), male rats were divided into 7 groups: Sham control (SC); RES vehicle (RV); RES (R); STZ‐diabetic (D; induced at 30dpp with 65 mg/kg of streptozotocin); STZ‐diabetic + insulin (DI); STZ‐diabetic + RES (DR); STZ‐diabetic + insulin +RES (DIR). DR, DIR, and R groups received 150mg RES/kg b.w./day by gavage (from 33 to 110dpp). DI and DIR received insulin (from day 5 after DM1 induction until 110dpp). Blood glucose was monitored in different time points. Animals were mated with healthy females. Euthanasia occurred at 110 dpp. Results DM1 increased lipid peroxidation (testis and epididymis) and sperm DNA fragmentation, alterations of chromatin structure, reduced mitochondrial mass and acrosome integrity, causing a decline in fertility and pregnancy rates. RES improved the parameters. Discussion RES, as an adjuvant, activates specific reactions against hyperglycemia, the main trigger of most complications of diabetes, by controlling oxidative stress, probably as a result of SIRT1 activation. We present here more evidences showing its valuable role in diminishing diabetes seriousness to male reproduction, not only to spermatogenesis in the first instance, but also to sperm overall quality and fertility outcomes, regardless of insulin treatment. Conclusion RES attenuated lipid peroxidation and sperm DNA damage in DM1‐induced animals, which positively reflected on male fertility. Our results show RES potential against DM1 complications in male reproduction.

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Contemporaneous effects of diabetes mellitus and hypothyroidism on spermatogenesis and immunolocalization of Claudin-11 inside the seminiferous tubules of mice

Cited by 7 publications

References 53 publications

Impact of Quercus infectoria Galls Extract on Thyroid Gland and Testicular Functions in Diabetic Rats

Impact of Quercus infectoria Galls Extract on Thyroid Gland and Testicular Functions in Diabetic Rats

Resveratrol Protects against Restraint Stress Effects on Stomach and Spleen in Adult Male Mice

Resveratrol improves sperm DNA quality and reproductive capacity in type 1 diabetes

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