The syndrome of perifollicular fibromas and colonic polyps was delineated 20 years ago by Hornstein and Knickenberg; it probably occurs more frequently than suggested by the literature. Multiple perifollicular fibromas were found in a mother and daughter. The mother also had colonic polyps. This dermo-intestinal syndrome varies in its clinical manifestations, but it is probably an autosomal dominant trait. We believe that the Horn-stein-Knickenberg syndrome and the Birt-Hogg-Dubé syndrome are identical. If perifollicular fibromas are observed and cannot be explained as postinflammatory sequelae of acne, the patient should be examined for colonic polyps as an appropriate from of cancer screening.
Photoinduced modifications of NAD(P)H attributed autofluorescence of CHO cells in asingle-beam gradient force optical trap (optical tweezers) were studied. Fluorescence spectra of single cells in the optical trap were measured using a modified microscope with an JR microbeam at 1064 and 760 nm for trapping, UVA radiation at 365 nm for fluorescence excitation, and an optical multichannel analyzer for spectral recording. No strong effect of the 1064 nm trapping beam on fluorescence intensity and spectral characteristics was found, even for power densities up to 70 MW/cm2. In contrast, 760 nm microirradiation resulted in a significant fluorescence increase, probably indicating cell damage due to absorption by heme-containing molecules. UVA exposure (1 W/cm2) of the trapped cells generated within seconds an initial fluorescence decrease, followed by a significant increase up to 5X of the value prior to irradiation. The UVA-induced modifications reflect NAD(P)H autooxidation and irreversible cell damage due to oxidative stress.
The study of energy pools and dynamics of specific pathways in living cells by microspectrofluorometry and fluorescence imaging produces spectral and topographic images characterizing structural and functional changes associated with cytopathology. Microspectro-fluorometry and fluorescence imaging have been applied, together with organelle morphometry to a number of cells mimicking certain cytopathologies, including melanoma cells, long-term malignant cells, and gene-defective cells. These investigations of cellular pathology indicate that there is a convergence of various physiopathological processes. Cellular states that have similarities include senescence, detoxification, and transformation. While the NAD(P)H metabolic transients have been studied before, our emphasis in this article is on very rapidly scanned fluorescence images related to organelle integration and photoinduced cellular senescence.
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