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FAILURE to diagnose gonorrhea in women who harbor asymptomatic infection is a significant handicap in the control of gonorrhea. This handicap would be partially alleviated if a culture method could be made readily available to all physicians and clinics. Similar considerations apply to detection of nasopharyngeal carriers of meningococci in confined populations.A selective medium for the cultivation of gonococci and meningococci was reported by Thayer and Martin in 1964 (1). An improved medium was described by the same authors in 1966 (2). The Thayer-Martin selective medium is recommended for primary isolation of the gonococcus and meningococcus, especially from sites where these organisms are outnumbered by the more rapid-growing natural bacterial flora. Overgrowth by gram-positive and other gram-negative bacteria and yeast is prevented in most cases because the medium contains 3 units of vancomycin, 7.5 micrograms of colistimethate sodium, and 12.5 units nystatin per milliliter. Although this selective medium has been available since 1964, its use, unfortunately, is limited to

The in vitro action of nine antibiotics was tested by the agar streak method against 45 gonococcal strains isolated from penicillin-therapy failures. The penicillin susceptibility range of these strains was 0.003 to 1.32 μg/ml, and the tetracycline susceptibility range was 0.125 to 2.0 μg/ml. Minimal inhibitory concentrations of minocycline and doxycycline paralleled the activity of tetracycline and ranged from 0.125 to 1.0 μg/ml and 0.125 to 2.0 μg/ml, respectively. Rifampicin, with a narrow range of 0.5 to 1.0 μg/ml, inhibited 75% of the strains at 0.5 μg/ml. The range for cephaloridine and cephaloglycine was 0.5 to 20.0 μg/ml, but another cephalosporium derivative, cephalexin, exhibited greater activity in its range of 0.25 to 20.0 μg/ml. A semisynthetic penicillin, carbenicillin, with a range of 0.025 to 0.75 μg/ml, displayed more activity against the lower susceptible penicillin G gonococcal strains.

The in vitro action of nine antibiotics was tested by the agar streak method against 45 gonococcal strains isolated from enicillin-therapy failures. The illin susceptibility range of these strains was 0.003 to 1.32 jg/nl, and the tetracycline susceptibility range was 0.125 to 2.0 jg/ml. Minimal inhibitory concentrations of minocycline and doxycycline paralleled the activity of tetracycline and ranged from 0.125 to 1.0 jg/ml and 0.125 to 2.0 jLg/mnl respectively. Rifampicin, with a narrow range of 0.5 to 1.0 jig/ml, inhibited 75% of the strains at 0.5 jg/ml. The range for cephaloridine and cephaloglycine was 0.5 to 20.0 jg/ml, but another cephalosporium derivative, cephale exhibited greater activity in its range of 0.25 to 20.0 jig/ml. A smisynthetic piln, E lin with a range of 0.025 to 0.75 jg/ml, displayed more activity against the lower susceptible penicillin G gonococcal strains.