A model immunoassay between concanavalin A (antibody) and yeast mannan (antigen) was investigated using a microcomputer-controlled flow injection analysis (FIA) manifold with turbidimetric detection at 400 nm. The automated injection procedure gave good precision for a turbidimetric method and the stop-flow merging zones technique gave an acceptable sample throughput (50 samples per hour) with minimum consumption of sample (30 vl). The system described could therefore be used routinely for immunoprecipitin analysis in clinical laboratories, e.g., IgG in human serum, and also to study kinetic aspects of such reactions.
An immunological reaction between human serum immunoglobulin G (IgG) and goat anti-human IgG was investigated using a fully automated stop-flow merging zones flow injection analysis manifold. Turbidimetric detection at 340 nm was used to monitor the rate of reaction. A sampling rate of 40 samples per hour and a precision of 2.O-6.8% RSD (relative standard deviation) were obtained for a range of human serum standards. Serum samples and a human reference serum were analysed and their IgG concentrations interpolated from a second-order fit of the calibration data. The consumption of expensive antiserum was less than 1 pI per assay.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.