Determination of human serum immunoglobulin G using flow injection analysis with rate turbidimetric detection

Worsfold, Paul J.; Hughes, Arwel; Mowthorpe, D. J.

doi:10.1039/an9851001303

Cited by 26 publications

(8 citation statements)

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“…An immunological reaction between human serum immunoglobulin G (IgG) and goat anti-human IgG was developed using automated stopflow merging zones FIA manifolds by Worsfold et al Turbidimetric detection was used to monitor the rate of reaction. [74,75] Serum samples and human reference serum were analyzed and their IgG concentrations interpolated from a second-order fit [75] or from the linear [76] calibration data. In order to enhance the formation of large molecular aggregates and to increase the sensitivity, polyethylene glycol was introduced to the carrier stream.…”

Section: Immunologic Reactionsmentioning

confidence: 99%

Turbidimetric and Nephelometric Flow Analysis: Concepts and Applications

Morais

Tóth

Rangel

2006

Spectroscopy Letters

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A review on flow analysis with turbidimetric and nephelometric detection is presented. A brief discussion of the principles of turbidimetry and nephelometry is given. Particular emphasis is devoted to coupling different flow techniques (flow injection, sequential injection, multicommutation) to these detection techniques. Applications in environmental, pharmaceutical, biological, and food samples are summarized and compared in terms of application range, flow configuration, repeatability, and sampling rate.

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Section: Immunologic Reactionsmentioning

confidence: 99%

Turbidimetric and Nephelometric Flow Analysis: Concepts and Applications

Morais

Tóth

Rangel

2006

Spectroscopy Letters

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“…This contribution corrects for previous similar treatments derived by considering detectors with infinite sensitivity. A stop-flow, merging-zones, unsegmented-continuous-flow manifold and turbidimetric detection of rate have been used by Worsfold et al (271) for the determination of human serum immunoglobulin G. Turbidimetric monitoring is at 340 nm and the immunoglobulin concentration is interpolated from a second-order fit of calibration data. The reaction rate is measured by stopping a segment of the merged serum and antiserum zones in the flow cell after 14 s of sample injection and measuring the turbidity 30 and 60 s after stopping the zone.…”

Section: Miscellaneous Kinetic Aspects Of Analytical Interestmentioning

confidence: 99%

Kinetic determinations and some kinetic aspects of analytical chemistry

Mottola¹,

Pérez‐Bendito²,

Mark³

1988

Anal. Chem.

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“…A different approach is the measurement of IgG through the formation of immuno precipitates between antibody and antigen (Worsfold et al, 1985). Freitag et al (1991) adapted this method for monitoring online the concentration of pullulanase and anti-thrombin III.…”

Section: Introductionmentioning

confidence: 99%

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et al. 1997

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The monoclonal-antibody production of an immobilized hybridoma cell line cultivated in a fluidized-bed reactor was monitored on-line for nearly 900 h. The monoclonal antibody concentration was determined by an immuno affinity-chromatography method (ABICAP). Antibodies directed against the product, e.g. IgG, were immobilized on a micro-porous gel and packed in small columns. After all IgG present in the sample was bound to the immobilized antibodies, unbound proteins were removed by rinsing the column. Elution of the bound antibodies followed and the antibodies were determined by fluorescence. The analytical procedure was automated with a robotic device to enable on-line measurements. The correlation between the on-line determined data and antibody concentrations measured by HPLC was linear.A sampling system was constructed, which was based on a pneumatically actuated in-line membrane valve integrated into the circulation loop of the reactor. Separation of the cells from the sample stream was achieved by a depth filter made of glass-fibre, situated outside the reactor. Rapid obstruction of the filter by cells or cell debris and contamination of the sample system was avoided by intermittent rinsing of the sample system with a chemical solution. The intermittent rinsing of the filter, which had a surface of 4.8 cm(2), resulted in an operational capacity of up to 40 samples (1.0 l total sample volume). Both the sampling system and the analytical device functioned without failure during this long-term culture.The culture temperature was varied between 34 and 40 °C. Raising the temperature from 34 up to 37 °C resulted in a simultaneous increase of growth and specific antibody production rate. Specific metabolic rates of glucose, lactate, glutamine and ammonium stayed constant in this temperature range. A further enhancement of temperature up to 40 °C had a negative effect on the growth rate, whereas the specific monoclonal antibody production rate showed a small increase. The other specific metabolic rates also increased in the temperature range between 38 to 40 °C.

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Determination of human serum immunoglobulin G using flow injection analysis with rate turbidimetric detection

Cited by 26 publications

References 0 publications

Turbidimetric and Nephelometric Flow Analysis: Concepts and Applications

Turbidimetric and Nephelometric Flow Analysis: Concepts and Applications

Kinetic determinations and some kinetic aspects of analytical chemistry

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