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Islet cells transplantation has limitations like low survivability, which can be overcome by using extracellular matrix mimicking three-dimensional (3D) scaffolds, which supports the growth and proliferation of seeded cells. This study was aimed to investigate the role of novel 3D carboxymethyl guargum (CMGG) nanocomposite with reduced graphene oxide (rGO) for proliferation of pancreatic islet cells (RIN-5F) and rate of insulin secretion of RIN-5F cells. Scanning electron microscope and Fourier transform infrared results have demonstrated good porosity and the chemical interactions between CMGG and rGO. Mechanical testing and thermogravimetric analysis of nanofibers have shown good tensile strength and thermal stability with rGO in the nanocomposite. These scaffolds demonstrated in vitro biocompatibility with acceptable ranges of biodegradability and hemocompatibility. The in vitro cell proliferation and viability of RIN-5F cells on 3D CMGG nanofibers have significantly increased compared to two-dimensional (2D) cell control. Moreover, the glucose dependent insulin secretion of RIN-5F cells on CMGG nanocomposite has significantly increased upto 4–5 folds than cells on 2D cell control. The biomaterials used in this 3D nanofiber scaffold have shown to be biodegradable and hemocompatible and can be a promising platform for the proliferation and secretion of insulin from beta cells and can be effectively used in transplantation type-1 diabetes.

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