Adenylate energy charge (AEC) has been used as a practical index of the
physiological status and health in several disciplines, such as ecotoxicology
and aquaculture. This study standardizes several procedures for AEC
determination in penaeid shrimp that are very sensitive to sampling. We
concluded that shrimp can be frozen in liquid nitrogen and then stored at
-76?C for up to two years for further analysis, or freshly dissected and
immediately homogenized in acid. Other cooling procedures, such as immersion
in cold water or placing shrimp on ice for 15 min resulted in 50% and 73%
decreases in ATP levels, and 9-fold and 10-fold increases in IMP levels,
respectively. Optimal values of AEC (0.9) were obtained in shrimp recently
transferred from ponds to indoor conditions, but decreased to 0.77 after one
month in indoor tanks when stocked at high densities; the AEC re-established
to 0.85 when the shrimps were transferred to optimal conditions (lower
density and dark tanks). While the levels of arginine phosphate followed the
same pattern, its levels did not fully re-establish. Comparison of different
devices for sample homogenization indicated that a cryogenic ball mill mixer
is the more suitable procedure.
In the mitochondrial F(O)F(1) ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei and their phylogenetic relationships. We also investigated the effect of hypoxia on shrimp by measuring changes in the mRNA amounts of atpα and atpβ. Our results confirmed highly conserved regions for both subunits and underlined unique features among others. The ATPβ deduced protein of shrimp was less conserved in size and sequence than ATPα. The relative mRNA amounts of atpα and atpβ changed in shrimp pleopods; hypoxia at 1.5 mg/L caused an increase in atpβ transcripts and a subsequent decrease when shrimp were re-oxygenated. Results confirm that changes in the mRNAs of the ATP-synthase subunits are part of the mechanisms allowing shrimp to deal with the metabolic adjustment displayed to tolerate hypoxia.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.