Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei FOF1 ATP-synthase complex: cDNA sequences, phylogenies, and mRNA quantification during hypoxia

Martínez‐Cruz, Oliviert; García‐Carreño, Fernando; Robles-Romo, Arlett; Varela-Romero, Alejandro; Muhlia‐Almazán, Adriana

doi:10.1007/s10863-011-9340-0

Cited by 18 publications

(13 citation statements)

References 42 publications

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“…As a novel cell death regulator, subunit β (AtpB) plays a pivotal role in stress responses. Up-regulation of AtpB transcription marks initiation of the aging process [21,22]. Complex V cannot be assembled successfully and respiration rates are reduced resulting in inhibition of ATP synthesis in the absence of the β subunit [21].…”

Section: Introductionmentioning

confidence: 99%

Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Qian

et al. 2013

BMC Plant Biol

126

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BackgroundRecent studies have demonstrated that cellular energy is a key factor switching on ripening and senescence of fruit. However, the factors that influence fruit energy status remain largely unknown.ResultsHPLC profiling showed that ATP abundance increased significantly in developing preharvest litchi fruit and was strongly correlated with fruit fresh weight. In contrast, ATP levels declined significantly during postharvest fruit senescence and were correlated with the decrease in the proportion of edible fruit. The five gene transcripts isolated from the litchi fruit pericarp were highly expressed in vegetative tissues and peaked at 70 days after flowering (DAF) consistent with fruit ADP concentrations, except for uncoupling mitochondrial protein 1 (UCP1), which was predominantly expressed in the root, and ATP synthase beta subunit (AtpB), which was up-regulated significantly before harvest and peaked 2 days after storage. These results indicated that the color-breaker stage at 70 DAF and 2 days after storage may be key turning points in fruit energy metabolism. Transcript abundance of alternative oxidase 1 (AOX1) increased after 2 days of storage to significantly higher levels than those of LcAtpB, and was down-regulated significantly by exogenous ATP. ATP supplementation had no significant effect on transcript abundance of ADP/ATP carrier 1 (AAC1) and slowed the changes in sucrose non-fermenting-1-related kinase 2 (SnRK2) expression, but maintained ATP and energy charge levels, which were correlated with delayed senescence.ConclusionsOur results suggest that senescence of litchi fruit is closely related with energy. A surge of LcAtpB expression marked the beginning of fruit senescence. The findings may provide a new strategy to extend fruit shelf life by regulating its energy level.

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Section: Introductionmentioning

confidence: 99%

Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Qian

et al. 2013

BMC Plant Biol

126

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“…In shrimp the complete sequence of ATP2 has been published [14], [8] but not that of ATP3 . We therefore employed a BLAST search tool using ATP3 gene from Drosophila melanogaster (accession number NM_079826 ) against the NCBI and Marine Genomics databases.…”

Section: Resultsmentioning

confidence: 99%

“…Only 4 have been reported in the Pacific white shrimp Penaeus vannamei . These are ATP1 (α subunit), ATP2 (β subunit), ATP6 (6 or a subunit), and ATP9 (9 or c subunit) [7],[8]. Here, we reported a novel cDNA sequence encoding an ATP3 gene (γ subunit) from P. vannamei and described the first attempt to use a yeast functional complementation assay for shrimp ATPase genes.…”

Section: Introductionmentioning

confidence: 99%

Shrimp ATP synthase genes complement yeast null mutants for ATP hydrolysis but not synthesis activity

et al. 2012

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The aim of this study was to examine the feasibility of employing a yeast functional complementation assay for shrimp genes by using the shrimp mitochondrial F1F0-ATP synthase enzyme complex as a model. Yeast mutants defective in this complex are typically respiratory-deficient and cannot grow on non-fermentable carbon sources such as glycerol, allowing easy verification of functional complementation by yeast growth on media with them as the only carbon source. We cloned the previous published sequence of ATP2 (coding for ATP synthase β subunit) from the Pacific white shrimp Penaeus vannamei (Pv) and also successfully amplified a novel PvATP3 (coding for the ATP synthase γ subunit). Analysis of the putative amino acid sequence of PvATP3 revealed a significant homology with the ATP synthase γ subunit of crustaceans and insects. Complementation assays were performed using full-length ATP2 and ATP3 as well as a chimeric form of ATP2 containing a leader peptide sequence from yeast and a mature sequence from shrimp. However, the shrimp genes were unable to complement the growth of respective yeast mutants on glycerol medium, even though transcriptional expression of the shrimp genes from plasmid-borne constructs in the transformed yeast cells was confirmed by RT-PCR. Interestingly, both PvATP2 and PvATP3 suppressed the lethality of the yeast F1 mutants after the elimination of mtDNA, which suggests the assembly of a functional F1 complex necessary for the maintenance of membrane potential in the ρ0 state. These data suggest an incompatibility of the shrimp/yeast chimeric F1-ATPase with the stalk and probably also the F0 sectors of the ATP synthase, which is essential for coupled energy transduction and ATP synthesis.

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“…35,36 For this instances adult L. vannamei shrimp weighing 30 ± 1 g were acclimatized to laboratory Mitochondrial protein contents were determined by the Biuret reaction. In this regard, mitochondria from hepatopancreas of Litopenaeus vannamei were isolated by standard differential centrifugation.…”

Section: Litopenaeus Vannamei Exposed To Carbon Nanotubesmentioning

confidence: 99%

“…In this regard, mitochondria from hepatopancreas of Litopenaeus vannamei were isolated by standard differential centrifugation. 35,36 It were performed respiration protocols using three types of multi-walled carbon nanotubes MWCNT which were formed by 3 concentric tubes with distance of 0.34 nm between each wall and semiconducting behavior (conductivity = 100 Scm -1 ). 35,36 Continuous-monitoring of oxygen consumption in mitochondrial suspensions was polarographically determined with a Clark-type electrode (Oxygraph System Hansatech Instrumens) in a 2 ml glass chamber equipped with a magnetic stirrer.…”

Section: Litopenaeus Vannamei Exposed To Carbon Nanotubesmentioning

confidence: 99%

Predicting the binding properties of single walled carbon nanotubes (SWCNT) with an ADP/ATP mitochondrial carrier using molecular docking, chemoinformatics, and nano-QSBR perturbation theory

et al. 2016

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Interactions between single walled carbon nanotubes (SWCNT) family with mitochondrial ADP/ATP carrier (ANT-1) were evaluated using constitutional (functional groups, number of carbon atoms, etc.) and electronic nanodescriptors defined by (n, m)-Hamada indexes (armchair, zig-zag and chiral). The Free Energy of Binding (FEB) was determined by Molecular Docking Simulation and the results showed that FEB was statistically more negative (p< 0.05), following the order SWCNT-COOH > SWCNT-OH > SWCNT, suggesting that polar groups favor the anchorage to ANT-1. In this regard, it was showed that key ANT-1 amino acids (Arg 79, Asn 87, Lys 91, Arg 187, Arg 234 and Arg 279) responsible for ADP-transport were conserved in ANT-1 from different species examined to predict SWCNT interactions, including shrimp Litopenaeus vannamei and fish Danio rerio commonly employed in ecotoxicology. The SWCNT-ANT-1 inter-atomic distances for the key ANT-1 amino acids were similar to that with carboxyatractyloside, a classical inhibitor of ANT-1.Significant linear relationships between FEB and n-Hamada index were found for zig-zag SWCNT and SWCNT-COOH (R 2 = 0.95 in both cases). A Perturbation Theory-Nano-Quantitative Structure-Binding Relationship (PT-NQSBR) model was fitted that was able to distinguish between strong (FEB < -14.7 kcal/mol) from weak (FEB ≥ -14.7 kcal/mol) SWCNT-ANT-1 interactions. A simple ANT-1-inhibition respiratory assays employing mitochondria suspension from L. vannamei, showed good accordance with the prediction model. These results indicate that this methodology can be employed in massive virtual screenings and used for making regulatory decisions in Nanotoxicology.

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Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei FOF1 ATP-synthase complex: cDNA sequences, phylogenies, and mRNA quantification during hypoxia

Cited by 18 publications

References 42 publications

Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Shrimp ATP synthase genes complement yeast null mutants for ATP hydrolysis but not synthesis activity

Predicting the binding properties of single walled carbon nanotubes (SWCNT) with an ADP/ATP mitochondrial carrier using molecular docking, chemoinformatics, and nano-QSBR perturbation theory

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