Ralstonia solanacearum is a tomato pathogen causing wilt disease which can lose crop production up to 100%. This problem also has economical impact to Indonesia, though controlling this pathogen has been done before. Biological control using endophytic bacteria is one of alternative control methods to support agriculture sustainability. The objective of these experiments are isolating endophytic bacteria taken from healthy tomato stems and root as well as investigating patogens as biocontrol agents of tomato bacterial wilt disease. Among 10 isolates found, only Ps1 and Ps8 can inhibit R. solanacearum based on antagonistic test in vitro using seed coat method and 4-7 mm inhibition zone forming. In vivo test was applied in green house in 30 days old of tomatoes using endophytic bacteria by the root soak technique. The result shows Ps1 and Ps8 within 15-16 days incubation period have suppressed 8.07%-9.19% pathogen attack.
<p>Wilt disease of <em>Solanaceae</em> caused by <em>Ralstonia solanacearum</em> reduce the crop quality and negatively affect the crop product. The objective of this research was to discover of endophytic bacteria formulation that effectively decreases bacterial which cause wilt disease on <em>Solanaceae</em>. The research consisted of purification of <em>Ralstonia solanacearum</em>, endophytic bacteria were obtained from the sample and the screening of endophytic bacteria using this following assay: antagonist assay, seedling assay and <em>in planta</em> assay. The results showed that in antagonist assay, the bacterial isolate code PS<sub>1</sub>, PS<sub>2</sub>, and PS<sub>8 </sub>could inhibit growth of <em>R. solanacearum. </em>From the seedling assay, it obtained that all of the isolates increased of percentage of germination, seed coating and powder formulation can decrease disease incidence of bacterial wilt disease.</p>
Potential of Seaweed in the Inhibition of Fungi Growth Aspergillus flavus. This use of synthetic chemicals as a controller of fungal growth in foodstuffs can have adverse effects on health, so it needs natural control ingredients that do not have an impact on human health. Various types of fungi such as Aspergillus flavus attack a lot of food items after harvest, these fungi can produce aflatoxin which is very toxic to consumers. Aflatoxin cannot be neutralized through cooking so efforts to avoid fungal contamination need to be done. One of the natural fungi controllers is by utilizing natural resources, including seaweed. Development of seaweed as an antifungal is an alternative that needs to be realized. For this reason, it is necessary to examine the potential inhibitory of seaweed A. flavus development. This study examined six types of seaweed like Gracilaria verrucosa, Euchema spinosum, Ulva lactuca, Ulva reticulata, Euchema edule, and Padina sp. The results showed that Padina sp. seaweed has a very strong potential inhibitory compared to other types of seaweed.
Escherichia coli is a bacteria found in the large intestine of humans as a normal flora. It’s unique because it can cause primary infection in the intestine such as diarrhea in children. Diarrhea sufferers use drugs derived from chemicals and herbal plants. Furthermore seaweed as the marine biological resources it is important to examine to be an alternative treatment for diarrhea. An antibacterial alternative by development the use of seaweed. needs to be examine. Based on the description above, phytochemical compound of several types of green macro algae and its potential effect as antibacterial activity to inhibit the growth of E.coli bacteria it is necessary to examine, The type of seaweeds that use in this study are Ulva reticulata, Ulva lactuca and Caulerpa racemosa. The research results showed that phytochemcal compound of U. reticulata are terpenoid, phenolic, alkaloid. In U. lactuca are steroid, saponin, phenolic and alkaloid. Phytochemcal compound of C. racemosa are phenolic, flavonoid, alkaloid, and terpenoid. Type of green macro algae U. reticulata had the ability to inhibit the bacteria E.coli growth with a inhibition zone diameter about 6.0 mm U. lactuca about 10 mm, and C. racemosa about 12.0 mm.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.