Polynucleobacter difficilis sp. nov., a planktonic freshwater bacterium affiliated with subcluster B1 of the genus Polynucleobacter

No abstract

In this work the effect of 4Hz 30dB horizontal mechanical vibration (MV) on thermal pain threshold, hydration and [3 H]-ouabain binding in brain and heart muscle tissues of rats was studied. It was revealed that 4Hz MV treatment for 10 minutes increased pain threshold, which was accompanied by brain and heart muscle tissue dehydration. In vitro state, hydration of brain and heart muscle tissues of sham animals was increased, while in 4Hz MV-treated animals the increase of brain tissue hydration was more pronounced and heart muscle tissues were dehydrated. The fact that 4Hz MV treatment also impacted heart muscle tissue hydration indicates that 4Hz MV effect on brain and heart muscle tissues is realized through a common messenger circulating in blood. The incubation of brain and heart muscle tissues in PS containing 10 -4 M and 10 -9 M ouabain led to tissue hydration in sham and 4Hz MV-treated animals. However, tissues of 4Hz MV-treated animals were less hydrated, and this hydration was accompanied by the decrease and increase of membrane receptors' affinity at 10 -4 M and 10 -9 M ouabain concentrations, respectively. Based on the obtained data, it is suggested that pain-relieving effect of 4Hz MV is due to α3 isoform-dependent brain tissue dehydration.

Haematococcus pluvialis is a genus of green algae that keeps the high interest of the scientists for decades due to its capacity to synthesize astaxanthin. Among many others, astaxanthin is one of the most demanded natural anti-oxidant and the product of the future. It has the widest usage as source of nutrient rich food and health promoting compounds. Astaxanthin has got various applications in the nutraceuticals as immune system enhancer and anti-cancer agent, in the cosmetics as anti-aging component, as additive to food, as well as in the aquaculture industries. As a key requirement for the research in this field is to find out the better conditions and media to stimulate the H.pluvialis higher growth rate to reach in the field of microalgae economically sustainable cultivation and processing. The effect of WC versus BG-11 media to enhance growth rate of Haematococcus pluvialis has been examined. This study employed a parallel testing of stimulating effect of extra vitamins versus phosphorus. Combination of mentioned parameters with gentle shacking have been determined as multiple effectors that contribute to green algae cultures to grow to high density. Importantly, this study demonstrates that extra phosphorus contribute to growth rate of H.pluvilais positively, meanwhile addition of extra vitamins in combination with extra phosphorus impact both positively and negatively to promote cells division. Shacking influences the culture growth as either stimulator or repressor.