Stimulated Growth of Green Algae Haematococcus Pluvialis Under Different Environmental Conditions

Abstract

Haematococcus pluvialis is a genus of green algae that keeps the high interest of the scientists for decades due to its capacity to synthesize astaxanthin. Among many others, astaxanthin is one of the most demanded natural anti-oxidant and the product of the future. It has the widest usage as source of nutrient rich food and health promoting compounds. Astaxanthin has got various applications in the nutraceuticals as immune system enhancer and anti-cancer agent, in the cosmetics as anti-aging component, as additive to food, as well as in the aquaculture industries. As a key requirement for the research in this field is to find out the better conditions and media to stimulate the H.pluvialis higher growth rate to reach in the field of microalgae economically sustainable cultivation and processing. The effect of WC versus BG-11 media to enhance growth rate of Haematococcus pluvialis has been examined. This study employed a parallel testing of stimulating effect of extra vitamins versus phosphorus. Combination of mentioned parameters with gentle shacking have been determined as multiple effectors that contribute to green algae cultures to grow to high density. Importantly, this study demonstrates that extra phosphorus contribute to growth rate of H.pluvilais positively, meanwhile addition of extra vitamins in combination with extra phosphorus impact both positively and negatively to promote cells division. Shacking influences the culture growth as either stimulator or repressor.

Influence of culture medium on growth and protein production by Haematococcus pluvialis

“…The slowest growth was observed in BG-11, which had the highest exponential phase compared to other culture media, reaching the stationary phase after 14 days of cultivation (Figure 1). Minasyan (2018) also observed that cultivation of H. pluvialis in the BG-11 medium achieved greater density with a more gradual growth rate. Nahidian, Ghanati, Shahbazi, and Soltani (2018), evaluating the development of the four cellular stages of H. pluvialis during 35 days of cultivation, also observed a longer growth time in a BG-11 than BBM medium.…”