O objetivo do trabalho foi avaliar o crescimento da microalga Navicula sp. utilizando resíduo sólido de um cultivo em sistema de bioflocos como meio de cultura em comparação ao meio Conway. Foram realizados dois experimentos, com e sem adição de metais traços, sendo que cada experimento teve cinco tratamentos com três repetições cada um: R0C100 (100% Conway); R25C75 (25% resíduo e 75% Conway); R50C50 (50% resíduo e 50% Conway), R75C25 (75% resíduo e 25% Conway) e R100C0 (100% resíduo). Os cultivos de alga foram realizados em erlenmeyers de 1 L durante 10 dias, com fotoperíodo integral e inóculo inicial de 5x10 4 cél. mL-1. Realizaram-se contagens diárias para acompanhamento da densidade celular máxima, tempo de duplicação e velocidade de crescimento. O pH e a temperatura foram mensurados no início e no final dos experimentos. Para as análises estatísticas, utilizaram-se os testes de Cochran, Shapiro Wilk, ANOVA e Tukey (P < 0,05). O pH e a temperatura mantiveram-se dentro dos padrões de cultivo nos dois experimentos. O meio de cultura com resíduo de cultivo de camarão em biofloco apresentou resultado semelhante ao do meio Conway e mostrou-se satisfatório para o desenvolvimento da microalga Navicula sp., ressaltando que a presença de metais traços favoreceu o crescimento da espécie. Palavras-chave: tratamento de efluente; microalga; crescimento.
Proteins from microalgal biomass have become promising raw materials in several industrial segments. To evaluate growth performance and protein production under different nutritional conditions, Haematococcus pluvialis was grown in different culture media: BBM, RM, BG-11, and KM2. The cultures were inoculated at 105 cells mL-1, and submitted to a temperature of 24°C, in a continous photoperiod and irradiance of 40 µmol photons m-2 s-1. The highest cell density was observed when H. pluvialis was maintained in BG-11 medium (142 ± 30 × 104 cells mL-1), but no statistical difference was observed when comparing the results with those obtained when culturing this microalga in BBM (101 ± 14 × 104 cells mL-1) and RM (105 ± 5 × 104 cells mL-1) media. Also, the lowest cell density was found when cultivating H. pluvialis in KM2 medium (57 ± 9 × 104 cells mL-1), and there was no statistical difference for doubling time, growth rate and specific growth rate results between treatments. In addition, higher protein contents in H. pluvialis were reported for RM, BG-11, and KM2 culture media at 55.1 ± 5.6, 49.3 ± 3.6 and 58.4 ± 2.8%, respectively; and lower protein content was found using the BBM medium (31.1 ± 2.9%). The highest cell density and biomass were achieved at greater nitrogen availability and a higher nitrogen to phosphorus ratio. The results suggest that H. pluvialis is a potential species for protein production, and that BG-11 is the most suitable medium for growing this microalga as it allowed the achievement of highest biomass production and protein content among the media evaluated.
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