Background
Entada phaseoloides
is a well-known medicinal plant traditionally used in Ayurvedic medicine for centuries.
Objective
To evaluate the anti-stress activity of seeds of
E. phaseoloides
in endoplasmic reticulum stress during chronic restrain stress in mice, based on our preliminary screening.
Materials and Methods
Mice (n = 6/group) were restrained daily for 6 h in 50 ml polystyrene tubes for 28 days. Methanolic extract of
E. phaseoloides
(MEEP) (100 and 200 mg/kg, p.o.) and standard drug, imipramine (10 mg/kg i.p.) were administered daily 45 min prior to restrain from day 22–28. Then, forced swim test (FST) was performed to assess despair behavior. Lipid peroxidation (LPO) and antioxidant enzymes Reduced glutathione (GSH), Superoxide dismutase (SOD) were measured in the hippocampus of mice. 78 kDa Glucose-regulated Protein, 94 kDa Glucose-regulated Protein, C/EBP homologous protein, Caspase-12 expression were quantified by Real Time PCR.
Results
MEEP significantly reduced the immobility time in FST (
P
< 0.001). Significant reduction of LPO (
P
< 0.05) level and restored antioxidant enzymes viz. GSH (
P
< 0.001) and SOD towards vehicle control group were observed. Down-regulation of genes GRP 78, GRP 94 (
P
< 0.001), CHOP and Caspase-12 (
P
< 0.001) as compared to the chronic restrain stress group was evident, which were upregulated following treatment. Isolation of the active components of the seeds revealed the presence of Oleic acid (
1
), Entadamide A (
2
), Entadamide A-beta-
d
-glucopyranoside (
3
) and 1-O-protocatechuoyl-β-
d
-glucose.
Conclusion
MEEP altered endoplasmic reticulum stress in chronic restrain stressed mice; however, as an antidepressant it showed a weaker response.
Textile dyeing industries are one of the major culprits for environmental pollution. The industries are adopting various processes for the removal of dyes and chemicals from the effluent before disposing to the land or water bodies. In the reported study, biochars were prepared from almond, coconut, and mustard oil cakes by chemical activation with phosphoric acid followed by low temperature pyrolysis. The ball milling technique was employed to reduce the particle size of the biochars below 300 nm. The synthesized biochars were used for the removal of color from the acid dye effluent from the wool dyeing unit. The results showed that very small quantities (2.0 %) of biochars are sufficient to remove around 92% color from the dye effluent. The batch adsorption and kinetic studies indicate the highest efficiency of color removal for the biochar derived from almond oil cake, followed by mustard and coconut. The adsorption properties of the synthesized biochars were found to be greatly depending on the type of oil cake used. It is concluded that the biochars produced from the oil cakes may be a partial replacement of petroleum based activated carbon for the color removal from wool textile dye effluent.
The objective of this study was to use human held ketone meter for the estimation of β-hydroxybutyrate (ketone body) for the diagnosis of sub-clinical ketosis in goat. A total of 210 samples from pregnant and lactating doe in Guwahati, Assam and nearby area were collected in aseptic condition from the Jugular Vein. Goats having blood ketone level more than 0.4 mmol/l and glucose level less than 30 mg/dl were considered positive for sub-clinical ketosis. Total 24 goats were diagnosed subclinical ketotic.
Two CMS lines, IR 58025A and IR 68888A along with their maintainers and two fertility restorers, LuitR and IR 36R, were evaluated for flower and plant characters during early ahu (February-June) and kharif (July-November) seasons. IR 58025A showed longer stigmata and styles, and higher spikelet Length/Breadth (L/B) ratio while IR 68888A showed broader stigmata and wider glume opening angle in both the seasons. IR 68888A also exhibited higher pollen sterility during early ahu. IR 36R was characterized with broad anthers. LuitR showed longer and broader anthers with more pollen than others. Plant height, flag leaf length, flag leaf width and area were higher in IR 36R. Panicle exsertion was complete in pollen parents whereas it was 78-80% in CMS lines. The widest flag leaf angle was found in IR 58025B during early Ahu and in IR 36R during kharif. Kharif season was more favourable for growth of the plants with higher seed set percentage while floral traits of the CMS lines were better expressed in early Ahu. Manipulation of the seeding sequence of the parental lines in early Ahu is warranted for better seed set provided the seed crop escapes heavy premonsoon showers during reproductive stage. IR 68888A/LuitR was a good combination for pollen dispersal and seed setting.
Knitted fabrics are subjected to various wet-relaxation treatments, and their degrees and stability of flat set are studied. Of the various methods used to study the degree of set, curling couple merits consideration in view of its sensitivity.
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