The objective of this study was to use human held ketone meter for the estimation of β-hydroxybutyrate (ketone body) for the diagnosis of sub-clinical ketosis in goat. A total of 210 samples from pregnant and lactating doe in Guwahati, Assam and nearby area were collected in aseptic condition from the Jugular Vein. Goats having blood ketone level more than 0.4 mmol/l and glucose level less than 30 mg/dl were considered positive for sub-clinical ketosis. Total 24 goats were diagnosed subclinical ketotic.

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The potential production of β-glucosidase by new strain Aspergillus protuberus under solid state fermentation was studied. Cultural and nutritional factors affecting β-glucosidase production were also investigated in order to optimize the fermentation conditions for the maximization of production. Different fermentation parameters such as different solid substrates, carbon and nitrogen sources, initial pH, moisture content, temperature, and optimized medium on β-glucosidase production were investigated. Rice husk served as the best solid support for maximal production of β-glucosidase. A maximum β-glucosidase production 26.06 U/g of rice husk was achieved under optimal conditions such as 1% glucose, 2% ammonium sulfate, pH 3, 40% moisture content, 30 o C temperature. An approximately 6-fold increase in β-glucosidase production was achieved in the optimized medium as compared with the nonoptimized medium. These results indicate that β-glucosidase production could be improved using these kind of processes. Also, taking into consideration locally available cheap substrates, economic side of the route is justified.