ABSTRAKLatar Belakang: Kadar asam urat yang tinggi dalam darah disebut dengan kondisi hiperurisemia. Hiperurisemia diketahui dapat menyebabkan terbentuknya radikal bebas sehingga terjadi stress oksidatif dan mengakibatkan kerusakan hepatosit. Daun kemangi (Ocimum basilicum) diketahui memiliki kandungan antioksidan dan sifat hepatoprotektif yang dapat digunakan untuk terapi pencegahan kerusakan hepar akibat hiperurisemia. Metode: Penelitian ini merupakan penelitian experimental dengan post-test only controlled group design. Penelitian ini menggunakan sampel bahan biologi tersimpan dalam bentuk organ hepar mencit (Mus musculus) jantan galur swiss yang terbagi menjadi 4 kelompok perlakuan. Analisis data menggunakan uji statistik ANOVA dan perhitungan jumlah hepatosit mencit menggunakan aplikasi Image-J. Hasil: Terdapat perbedaan yang bermakna pada kelompok kontrol positif terhadap kelompok perlakuan 1 (KP1) (p=0,035), kelompok kontrol positif terhadap kelompok perlakuan 2 (KP2) (p=0,000), dan kelompok kontrol positif terhadap kelompok perlakuan 3 (KP3) (p=0,000). Kesimpulan: Pemberian ekstrak etanol daun kemangi (Ocimum basilicum) dapat menurunkan kerusakan hepatosit pada mencit yang diinduksi asam urat.Kata kunci: Ocimum basilicum, hepatosit, Mus musculus. ABSTRACTBackground: A high level of uric acid in the blood was called hyperuricemia. Hyperuricemia was known as the cause of the formation of free radicals that resulted in oxidative stress and hepatocyte damage. It was known that basil (Ocimum basilicum) leaves had antioxidant content and hepatoprotective properties which could be used to prevent liver damage due to hyperuricemia. Method: It was experimental study with post-test only controlled group design. Stored biological material samples in the form of liver organs of mice (Mus musculus) Swiss webster were used in this study which were divided into 4 treatment groups. The data were analyzed by using ANOVA statistical test. The Image-J application was used to calculate the number of hepatocytes of mice. Results: There were significant differences in the positive control group towards the first treatment group (KP1) (p = 0.035), positive control group towards the second treatment group (KP2) (p = 0.000), and positive control group towards the third treatment group (KP3) (p = 0.000). Conclusion: The effect of ethanol extract of basil (Ocimum basilicum) leaves can reduce hepatocyte damage on mice which were induced by uric acid.
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