Dengue disease is a global disease that has no effective treatment. The dengue virus (DENV) NS4B is a target for designing specific antivirals due to its importance in viral replication. Medicinal plants have been a savior for dengue virus as they consist of a class of phytochemicals having anti-viral activity and can pose a new approach ofstrong drug against viruses. The present study analyzes the activity of compounds against NS4B of DENV (1-4) serotypes. In this study Catechin, Cianidanol, Epicatechin, Eupatoretin, Glabranin, Laurifolin, DL-Catechin, astherapeutic agents were filtered by using Lipinski rule’s five and the drug-likeness property of these agents were used for assessment of pharmacological properties. The molecular docking results presented the 2-D structures of bioactive complex, which interacted with especially conserved residues of target domains. Interestingly, we find the Catechin, Laurifolin, Cianidanol have highest binding energy against NS4B in DENV-1,2,4 which is evident by the formation of more hydrogen bonds with the amino acid residues at the binding site of the receptor. Our results revealed that the bioactive compound, especially Catechin has significant anti-dengue activities. In addition, this study may be helpful in further experimental investigations.
Dengue outbreak has affected rural areas of Delhi-NCR, Haryana widely but it lacks in surveillance. High cases of dengue symptoms were reported in these regions whereas dengue symptoms have been a neglected issue in the anti-dengue drug development. Therefore, this study aims to analyze the status of the dengue infection, a rural issue of Delhi-NCR, Haryana and to identify the significance of dengue symptoms in anti-dengue drug development. The study was conducted when there is high chance of dengue infection i.e. from August 2015 to October 2015 at OPD Unit of PR Institute of Medical Science & Research, Delhi-NCR, Sonepat. It includes 158 patients from 24 rural areas of Haryana comprising both males and females from different age groups. Out of 20% cases, 6% were IgG-Positive, 9% were IgMPositive and 88% were NS1-Positive and rest 80% was normal. It includes 44% cases of thrombocytopenia. Badkhalsa village (28%), age group 18-24 (34%) and males (63%) reported cases of high infection. It was found that people with fewer platelet counts (Rai village) were not suffering from dengue whereas people with more platelet count reported dengue infection (Badkhalsa village). Interpretation & Conclusion: This study focuses on new research directions by highlighting the dengue symptoms importance in anti-dengue drug development also it is a first attempt to investigate the status of dengue, a rural issue of Delhi-NCR, Haryana and suggests that health authorities and people living in these regions should take initiatives for better health.
Background: Dengue is one of the most widespread arthropod-borne viral infections without any effective treatment. The anti-DENV-2 mechanism of plants Andrographis paniculata (whole plant), Tinospora cordifolia (stem & leaves), their bioactive synthetic compounds depend on acute febrile treatment, is poorly understood for new anti-dengue therapy development. Objectives: The current study was undertaken to evaluate in silico and in vitro study on crude extracts, bioactive fractions, bioactive synthetic compounds of A. paniculata, T. cordifolia against anti-DENV-2. Methods: In silico study was evaluated by Lipinski's rule of five, drug-likeness score and molecular docking against DENV-2 NS2B-NS3. After in silico study, the antiviral activity was performed under in vitro conditions with cytotoxicity, pre-incubation, post-incubation, and protective assay. Findings: It was observed that in in silico studies, the best docked compounds andrographolide (-11.58 kcal/mol), magnoflorine (-9.22 kcal/mol) and their combination (50:50); ethanolic extract of A. paniculata, aqueous-ethanolic (50:50) extract of T. cordifolia and their combination (50:50) extract, their bioactive fractions with possible phenolic glycosides, pyridinecarboxylic acid, flavone, phenols, phenylpropanoids, flavonoids, phenolic acid, alkaloids, isopalmitic acid, diterpenoids, quinic acid, isopalmitic acid and sesquiterpenoids compound class category, showed 50% minimum effective and inhibitory concentration. Conclusions:The crude extracts, bioactive fractions and bioactive synthetic compounds of A. paniculata and T. cordifolia and combination (50:50) could be the potential anti-DENV-2 therapy in in silico and in vitro infection model.
The emerging and re-emerging infectious diseases pose a threat to the global economy. The tendency to confront these pandemics is still questionable. In January 2020, the first case of coronavirus (COVID-19) in India was identified in Kerala. Later, the Indian government announced lockdown as a remedial action against COVID-19. The present work aims to elucidate the community behavioural response towards COVID-19 lockdown in India and presents comprehensive observations. Protection motivational theory has been used to build a conceptual framework. It was observed that community behavioural response includes maladaptive, adaptive and prosocial behaviours. In addition, we conclude that the effective strategy against the prevention, spread and control of pandemics and strategic management is still lacking. Hence, we propose a framework based on the learnings from COVID-19 to tackle future pandemics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.