In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview reporting in detail the different LAMP steps, focusing on designing and main characteristics of the primer set, different methods of result visualization, evolution and different application fields, reporting in detail LAMP application in plant virology, and the main advantages of the use of this technique.

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Among the 15 virus species detected in olive trees, Olive leaf yellowing associated virus (OLYaV) is found with high incidence and frequency in Southern Italy in particular. Effects of OLYaV on virgin olive oil yield and quality of Leccino and Ottobratica cultivars in the Calabria region (Southern Italy) are analyzed. Oil yield, free acidity, number of peroxides, spectrophotometric indices, total content of chlorophylls and carotenoids, total phenol content, composition of the fatty acids, total tocopherols, and total sterols content are determined on oil obtained from olive fruits collected in healthy/virus‐free and OYLaV‐infected trees. Almost all analyzed oil parameters are not statistically different with some exceptions. Oils derived from ‘Ottobratica’ OLYaV‐infected plants have free acidity significantly lower than oil from healthy plants. K232 of oil from virus‐free ‘Leccino’ trees is significantly lower than oil from infected trees. Even though some quality parameter differences between healthy and OLYaV‐infected oils are found, it is important to highlight that all oils can be considered in extra virgin olive oil category, within the UE maximum limit acceptance range. Results suggest a no negative interference by OYLaV in oil yield and quality, except for K232 values, whereas surprisingly suggest a positive effect of virus infection on free acidity parameter. Practical Applications: Based on the evidence that OYLaV does not interfere negatively in oil yield and quality parameters, it seems appropriate that the European Union Council Directives (2014/96/EU, 2014/97/EU, 2014/98/EU) introduced in the last olive certification scheme only ArMV, CLRV, and SLRSV. The application of compulsory EU directive, in Italy as in other countries, will improve the facilities of olive plants commercialization, guaranteeing enough their sanitary status. The findings here reported support the suggestion that the Italian Ministry of Agriculture could be less restrictive in voluntary Italian regulation (D.M. 20/11/2006). Olive leaf yellowing associated virus (OLYaV) is found with high incidence and frequency especially in Southern Italy. Effects of OLYaV on virgin olive oil yield and quality of Leccino and Ottobratica cultivars in the Calabria region (Southern Italy) are analyzed, including free acidity, number of peroxides, spectrophotometric indexes, total content of chlorophylls, carotenoids and phenol, composition of the fatty acids, total tocopherols and total sterols.