Impact of Olive Leaf Yellowing Associated Virus on Olive (<i>Olea europaea</i> L.) Oil

Fontana, Anna; Piscopo, Amalia; Bruno, Alessandra De; Tiberini, Antonio; Muzzalupo, Innocenzo; Albanese, G.

doi:10.1002/ejlt.201800472

Cited by 8 publications

(8 citation statements)

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“…The phytosanitary analyses showed that Apulian olive germplasm is in overall good phytosanitary status. OLYaV was the predominant viral agent, in accordance with the past evidences in Southern Italy (Fontana et al, 2019), while only seven genotypes were infected by CLRV. The sanitation treatments adopted resulted very effective in eliminating both viruses with 83% and 92% efficiency for in vivo and in vitro thermotherapy, respectively.…”

Section: Discussionsupporting

confidence: 87%

“…An essential prerequisite in the genetic resources' conservation is also the assessment of the phytosanitary status (Fontana et al, 2019). As for other vegetative propagated crops, olive is affected by several pathogens (viruses, fungi, bacteria and phytoplasmas) that persist in the budwood and can be transmitted and disseminated with it, with severe economic effects on yield and production quality (Martelli et al, 2002;Loconsole et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Re.Ger.O.P.: An Integrated Project for the Recovery of Ancient and Rare Olive Germplasm

et al. 2020

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The olive tree is one of the most important economic, cultural, and environmental resources for Italy, in particular for the Apulian region, where it shows a wide diversity. The increasing attention to the continuous loss of plant genetic diversity due to social, economic and climatic changes, has favored a renewed interest in strategies aimed at the recovery and conservation of these genetic resources. In the frame of a project for the valorization of the olive Apulian biodiversity (Re.Ger.O.P. project), 177 minor genotypes were recovered in different territories of the region. They were submitted to morphological, molecular, technological and phytosanitary status analysis in comparison with reference cultivars, then they were propagated and transferred in an ex situ field. All the available information was stored in an internal regional database including photographic documentation and geographic position. The work allowed obtaining information about the genetic diversity of Apulian germplasm, to clarify cases of homonymy and synonymy, to check the sanitary status, and to identify candidate genotypes useful both to set up breeding programs and to enrich the panel of olive cultivars available to farmers for commercial exploitation.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Re.Ger.O.P.: An Integrated Project for the Recovery of Ancient and Rare Olive Germplasm

et al. 2020

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“…Only a small quantity of the total phenols was transferred from drupes to the oils: EVO in fact possessed 0.03–1.15 g GA kg −1 , with higher results generally in the first production period. EVO of Ottobratica cv extracted in October were the samples with the highest TPC (1.15 g GA kg −1 ) among studied samples and with respect to other observations for the same cultivar and similar extraction processing 26–28 . Despite the varietal differences, this parameter can indeed vary among crop years, linked to the plant response toward environmental conditions 29,30 .…”

Section: Resultsmentioning

confidence: 51%

Antioxidant quantification in different portions obtained during olive oil extraction process in an olive oil press mill

et al. 2020

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BACKGROUNDDifferent antioxidant compounds are generally transferred from olives to olive oil during the production process. This work characterized the principal total bioactive compounds (tocopherols and phenols) in olives, olive oil and by‐products of four cultivars grown in Calabrian areas (southern Italy), considering the effect of harvesting period. Antioxidant capacity, total and individual phenolic compounds were also analysed.RESULTSDrupes, olive paste, pomace and olive wastewater showed similar phenolic compounds, while olive oil possessed a different composition, suggesting that phenols are not only transferred from drupe to oil, but also they change during oil production. Tocopherols varied among cultivars and harvesting period: generally, they were more abundant in samples produced in the first harvesting period.Qualitative and quantitative differences in phenolic composition and antioxidant activity were significantly found among cultivars in all the matrices.CONCLUSIONThe highest amount of total phenolic antioxidants ended up in olive wastewater with variability due to the olive cultivar, while only a small part of them finished in the oil. This work shows evidence that the availability of bioactive compounds in different portions from the olive oil extraction belong to different varietal origins. In particular, new information was acquired on Ottobratica Calipa, a new olive clone, that produced an olive oil with an interesting antioxidant amount. © 2020 Society of Chemical Industry

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“…A decrease in oil yield and maturity index in virus infected olives was also reported, as well as elevated total phenols in olive oil content from infected olives when compared to healthy fruits (Godena et al, 2012). Nevertheless, the Olive leaf yellowing associated virus (OLYaV) was not found to have a negative interference in oil yield and quality (Fontana et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Sensitive qPCR Methodology for Detection of the Olive-Infecting Viruses in Portuguese and Tunisian Orchards

et al. 2019

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Sensitive detection of viruses in olive orchards is actually of main importance since these pathogenic agents cannot be treated, their dissemination is quite easy, and they can have eventual negative effects on olive oil quality. The work presented here describes the development and application of a new SYBR ® Green-based real-time quantitative PCR (qPCR) analysis for specific and reliable quantification of highly spread olive tree viruses: Olive latent virus 1 (OLV-1) , Tobacco necrosis virus D (TNV-D), Olive mild mosaic virus (OMMV), and Olive leaf yellowing-associated virus (OLYaV). qPCR methodology revealed high specificity and sensitivity, estimated in the range of 0.8–8 copies of the virus genome, for the studied viruses. For validation of the method, total RNA and double strand RNA (dsRNA) from naturally infected trees were used. In a first trial, dsRNAs from trees of cv. “Galega vulgar” from a Portuguese orchard, were subjected to qPCR and from the 30 samples tested, 26 were TNV-D and/or OMMV-positive and 25 were OLV-1 positive. In a second trial, total RNA from trees of different cultivars from Tunisian orchards, were here tested by qPCR and all viruses were detected. From the 33 samples studied, the most prevalent virus detected in Tunisia orchards was OLV-1 (31 samples diagnosed), followed by OLYaV (20 samples diagnosed), and finally the combination in last TNV-D and/or OMMV (12 samples diagnosed). In both trials, qPCR demonstrated to be effective and sensitive, even when using total RNA as template. qPCR through the use of a SYBR ® Green methodology enabled, for the first time, a reliable, sensitive, and reproducible estimation of virus accumulation in infected olive trees, in which viruses are usually in low titres, that will allow gaining new insights in virus biology essential for disease control and give an important contribution for establishment of sanitary certification of olive propagative material.

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Impact of Olive Leaf Yellowing Associated Virus on Olive (Olea europaea L.) Oil

Cited by 8 publications

References 20 publications

Re.Ger.O.P.: An Integrated Project for the Recovery of Ancient and Rare Olive Germplasm

Re.Ger.O.P.: An Integrated Project for the Recovery of Ancient and Rare Olive Germplasm

Antioxidant quantification in different portions obtained during olive oil extraction process in an olive oil press mill

Establishment of a Sensitive qPCR Methodology for Detection of the Olive-Infecting Viruses in Portuguese and Tunisian Orchards

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