Aqueous dispersions of graphene oxide (GO) have been found to emit a structured, strongly pH-dependent visible fluorescence. Based on experimental results and model computations, this is proposed to arise from quasi-molecular fluorophores, similar to polycyclic aromatic compounds, formed by the electronic coupling of carboxylic acid groups with nearby carbon atoms of graphene. Sharp and structured emission and excitation features resembling the spectra of molecular fluorophores are present near 500 nm in basic conditions. The GO emission reversibly broadens and red-shifts to ca. 680 nm in acidic conditions, while the excitation spectra remain very similar in shape and position, consistent with excited state protonation of the emitting species in acidic media. The sharp and structured emission and excitation features suggest that the effective fluorophore size in the GO samples is remarkably well defined.
The sources of broad backgrounds in visible-near-IR absorption spectra of single-walled carbon nanotube (SWCNT) dispersions are studied through a series of controlled experiments. Chemical functionalization of nanotube sidewalls generates background absorption while broadening and red-shifting the resonant transitions. Extensive ultrasonic agitation induces a similar background component that may reflect unintended chemical changes to the SWCNTs. No major differences are found between spectral backgrounds in sample fractions with average lengths between 120 and 650 nm. Broad background absorption from amorphous carbon is observed and quantified. Overlapping resonant absorption bands lead to elevated backgrounds from spectral congestion in samples containing many SWCNT structural species. A spectral modeling method is described for separating the background contributions from spectral congestion and other sources. Nanotube aggregation increases congestion backgrounds by broadening the resonant peaks. Essentially no background is seen in sorted pristine samples enriched in a single semiconducting (n,m) species. By contrast, samples enriched in mixed metallic SWCNTs show broad intrinsic absorption backgrounds far from the resonant transitions. The shape of this metallic background component and its absorptivity coefficient are quantitatively assessed. The results obtained here suggest procedures for preparing SWCNT dispersions with minimal extrinsic background absorptions and for quantifying the remaining intrinsic components. These findings should allow improved characterization of SWCNT samples by absorption spectroscopy.
As opposed to inorganic counterparts, organic quantum dots often exhibit lower fluorescence efficiencies and are complex to synthesize. Here we develop nitrogen‐doped (N‐GQDs) and nitrogen–sulfur codoped (NS‐GQDs) graphene quantum dots exhibiting high‐yield visible and near‐IR emission that are synthesized via a single‐step microwave‐assisted hydrothermal technique with a single glucosamine‐HCl starting material (thiourea precursor used for NS‐GQDs). As‐synthesized N‐GQDs and NS‐GQDs are well‐dispersed (average sizes of 5.50 and 3.90 nm) with high crystallinity and pronounced G‐band. Formed by the bottom‐up assembly of glucosamine, they contain amine linkage and a variety of oxygen‐containing functional groups assessed by Fourier‐transform infrared spectroscopy with ≈2% sulfur for NS‐GQDs. The synthetic procedure allows varying their size and the bandgap. Unlike other graphene‐based quantum dots, these GQDs exhibit bright, stable fluorescence both in the visible and near‐IR with high quantum yields of up to 60%. Excitation‐dependent visible fluorescence is attributed to size‐dependent bandgaps, with near‐IR emission potentially arising from the emissive defect states/their arrangements. Advantageous properties of these GQDs are utilized to develop exciton recombination layer for organic light‐emitting devices exhibiting both photoluminescence and electroluminescence in the visible. Produced by ecofriendly one‐step scalable synthesis brightly‐emissive N‐GQDs and NS‐GQDs become a promising material for novel organic optoelectronics.
We investigate the gate field dependence of light absorption and emission of an individual, suspended semiconducting carbon nanotube using Raman and photoluminescence spectroscopies. We find a strong reduction in the absorption strength and a red shift of the E(33) state of the nanotube with increasing gate field. The photoluminescence from the E(11) state is quenched even stronger. We explain these observations in terms of field-doping and its effects on both the radiative and nonradiative decay rates of the excitons. Thus, gate field-induced doping constitutes an effective means of controlling the optical properties of carbon nanotube devices.
Despite significant advances of nanomedicine, the issues of biocompatibility, accumulation-derived toxicity, and the lack of sensing and in vivo imaging capabilities hamper the translation of most nanocarriers into clinic. To address this, we utilize nitrogen, boron/ nitrogen, and sulfur-doped graphene quantum dots (GQDs) as fully biocompatible multifunctional platforms allowing for multicolor visible/ near-IR imaging and cancer-sensing. These GQDs are scalably produced in one-step synthesis from a single biocompatible glucosamine precursor, are water-soluble, show no cytotoxicity at high concentrations of 1 mg/mL, and demonstrate substantial degradation at 36 h in biological environments as verified by TEM imaging. Because of their small sizes, GQDs exhibit efficient internalization maximized at 12 h followed by further degradation/excretion. Their high-yield intrinsic fluorescence in blue/ green and near-infrared allows for multicolor in vitro imaging on its own or in combination with other fluorophores, and offers the capabilities for in vivo near-IR fluorescence tracking. Additionally, nitrogen-and sulfur-doped GQDs exhibit pH-dependent fluorescence response that is successfully utilized as a sensing mechanism for acidic extracellular environments of cancer cells. It allows for the deterministic, ratiometric spectral discrimination between cancerous (HeLa and MCF-7 cell) versus healthy (HEK-293 cell) environments with substantial intensity ratios of 1.6 to 8. These results suggest fully biocompatible GQDs developed in this work as multifunctional candidates for in vitro delivery of active agents, multicolor visible/near-IR fluorescence imaging, and pH-sensing of cancerous environments.
Graphene oxide (GO), the most common derivative of graphene, is an exceptional nanomaterial that possesses multiple physical properties critical for biomedical applications. GO exhibits pH-dependent fluorescence emission in the visible/near-infrared, providing a possibility of molecular imaging and pH-sensing. It is also water soluble and has a substantial platform for functionalization, allowing for the delivery of multiple therapeutics. GO physical properties are modified to enhance cellular internalization, producing fluorescent nanoflakes with low (<15%) cytotoxicity at the imaging concentrations of 15 μg/mL. As a result, at lower flake sizes GO rapidly internalizes into HeLa cells with the following 70% fluorescence based clearance at 24 h, assessed by its characteristic emission in red/near-IR. pH-dependence of GO emission is utilized to provide the sensing of acidic extracellular environments of cancer cells. The results demonstrate diminishing green/red (550/630 nm) fluorescence intensity ratios for HeLa and MCF-7 cancer cells in comparison to HEK-293 healthy cells suggesting a potential use of GO as a non-invasive optical sensor for cancer microenvironments. The results of this work demonstrate the potential of GO as a novel multifunctional platform for therapeutic delivery, biological imaging and cancer sensing.
A new method is demonstrated for measuring the length distributions of dispersed single-walled carbon nanotube (SWCNT) samples by analyzing diffusional motions of many individual nanotubes in parallel. In this method, termed "length analysis by nanotube diffusion" (LAND), video sequences of near-IR fluorescence microscope images showing many semiconducting SWCNTs are recorded and processed by custom image analysis software. This processing locates the individual nanotubes, tracks their translational trajectories, computes the corresponding diffusion coefficients, and converts those values to nanotube lengths. The deduced length values are then compiled into a histogram of lengths present in the sample. By using specific excitation wavelengths and emission filters, this analysis is performed on selected (n,m) structural species. The new LAND method has been found to give distributions in very good agreement with those obtained by conventional AFM analysis of the same samples. Because it is fluorescence-based, LAND monitors only semiconducting, relatively pristine SWCNTs. However, it is less sensitive to artifacts from impurities and bundled nanotubes than AFM or light scattering methods. In addition, samples can be analyzed with less time and operator attention than by AFM. LAND is a promising alternative method for characterizing length distributions of SWCNTs in liquid suspension.
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