Isolation &identification of Newcastle disease virus from wild pigeonsinhabiting University of Baghdad (Al-Khwarizmi College of engineering)where velogenic and devastating infection of birds was encountered. Chickenembryos fibroblasts (CEF) were used for virus isolation (from infectedspleen and lungs) and propagation. Viral cytopathogenic effect was noticedon infected (CEF) cell culture within (24) hr post inoculation. The virus wasidentified by heamagglutination inhibition (HI) test using reference antiNDV serum.
Summary: The fluorescent antibody technique was evaluated for the first time as a diagnostic aid for the diagnosis of equine histoplasmosis. Sera were obtained from several clinical cases of epizootic lymphangitis. The globulin fraction was separated, quantitated and then labeled with fluoresceine isothiocyanate.
Several antigen fmtives were tried. Heat‐fixation proved to be the most appropriate. Conjugated sera diluted 1:5 yielded the highest fluorescence intensity with heat fixed mycelial antigen preparations. Acetone and p‐formaldehyde treated preparations resulted in less efficient intensity. Glutaraldehyde was the least effective fixative.
The test substantiated humoral antibody formation during the course of the infection and its results seem promising for its use as a diagnostic aid.
Zusammenfassung: Die Fluoreszenz Antikörpertechnik wurde erstmals als diagnostisches Hilfsmittel bei der Diagnose der Pferdehistoplasmose verwendet. Seren wwden von mehreren klinischen Fällen der epizootischen Lymphangitis gewonnen. Die Globulinfraktion wurde abgetrennt und mit Fluoresceinisothiocat markiert. Mehrere Fixierungsmethoden für das Antigen wurden untersucht. Die Hitzefixierung erwies sich als am günstigsten. Konjugierte Seren in einer Verdünnung von 1:5 ergaben die höchsten Fluoreszenzintensitäten mit hitzefixiertem Mgzelantigen. Aceton und Formaldehyd als Fixative ergaben weniger giinstige Resultate. Glutaraldehyd war am wenigsten wirksam. Mit dem Test ließ sich die Bildung humoraler Antikörper während des Verlaufs der Infektion nachweisen. Der Test stelit außerdem eine vielversprechende diagnostische Methode dar.
Pferdehistoplasmose
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