The ventrolateral preoptic area (VLPO) predominantly contains sleep-active neurons and is involved in sleep regulation. The perifornical-hypothalamic area (PF-HA) is a wake-regulatory region and predominantly contains wake-active neurons. VLPO GABAergic/galaninergic neurons project to the PF-HA. Previously, the specific contribution of VLPO neurons projecting to the PF-HA (VLPO > PF-HAPRJ) in sleep regulation in rats could not be investigated due to the lack of tools that could selectively target these neurons. We determined the contribution of VLPO > PF-HAPRJ neurons in sleep regulation by selectively activating them using designer receptors exclusively activated by designer drugs (DREADDs) in wild-type Fischer-344 rats. We used a combination of two viral vectors to retrogradely deliver the Cre-recombinase gene, specifically, in VLPO > PF-HA neurons, and further express hM3Dq in those neurons to selectively activate them for delineating their specific contributions to sleep–wake functions. Compared to the control, in DREADD rats, clozapine-N-oxide (CNO) significantly increased fos-expression, a marker of neuronal activation, in VLPO > PF-HAPRJ neurons (2% vs. 20%, p < 0.01) during the dark phase. CNO treatment also increased nonREM sleep (27% vs. 40%, p < 0.01) during the first 3 h of the dark phase, when rats are typically awake, and after exposure to the novel environment (55% vs. 65%; p < 0.01), which induces acute arousal during the light phase. These results support a hypothesis that VLPO > PF-HAPRJ neurons constitute a critical component of the hypothalamic sleep–wake regulatory circuitry and promote sleep by suppressing wake-active PF-HA neurons.
Neural oscillations of the mammalian olfactory system have been studied for decades. This research suggests they are linked to various processes involved in odor information analysis, depending on the vigilance state and presentation of stimuli. In addition, the effects of various anesthetics, including commonly used ones like chloral hydrate, pentobarbital,ketamine, and urethane, on the local field potential (LFP) in the olfactory bulb (OB) have been studied. In particular, the combination of xylazine and tiletamine–zolazepam has been shown to produce steady anesthesia for an extended period and relatively few adverse effects; however, their effects on the LFP in the OB remain unknown. To study those effects,we recorded the LFP in the OB of rats under xylazine–tiletamine–zolazepam anesthesia. During the period of anesthesia,the spectral powers of the 1–4, 9–16, 31–64, 65–90 frequency bands increased significantly, and that of 91–170 Hz frequency band decreased significantly, whereas no significant changes were observed in the 5–8 and 17–30 Hz ranges. These results reveal dynamic changes in the time and frequency characteristics of the LFP in the OB of rats under xylazine–tiletamine–zolazepam anesthesia and suggest that this combination of anesthetics could be used for studying oscillatory processesin the OB of rats.
The ventrolateral preoptic area (VLPO) contains GABAergic sleep-active neurons. However, the extent to which these neurons are involved in expressing spontaneous sleep and homeostatic sleep regulatory demands is not fully understood. We used calcium (Ca2+) imaging to characterize the activity dynamics of VLPO neurons, especially those expressing the vesicular GABA transporter (VGAT) across spontaneous sleep-waking and in response to homeostatic sleep demands. The VLPOs of wild-type and VGAT-Cre mice were transfected with GCaMP6, and the Ca2+ fluorescence of unidentified (UNID) and VGAT cells was recorded during spontaneous sleep-waking and 3 h of sleep deprivation (SD) followed by 1 h of recovery sleep. Although both VGAT and UNID neurons exhibited heterogeneous Ca2+ fluorescence across sleep-waking, the majority of VLPO neurons displayed increased activity during nonREM/REM (VGAT, 120/303; UNID, 39/106) and REM sleep (VGAT, 32/303; UNID, 19/106). Compared to the baseline waking, VLPO sleep-active neurons (n = 91) exhibited higher activity with increasing SD that remained elevated during the recovery period. These neurons also exhibited increased Ca2+ fluorescence during nonREM sleep, marked by increased slow-wave activity and REM sleep during recovery after SD. These findings support the notion that VLPO sleep-active neurons, including GABAergic neurons, are components of neuronal circuitry that mediate spontaneous sleep and homeostatic responses to sustained wakefulness.
A linear discriminant analysis transformation-based approach to the classification of three different motor imagery types for brain–computer interfaces was considered. The study involved 16 conditionally healthy subjects (12 men, 4 women, mean age of 21.5 years). First, the search for subject-specific discriminative frequencies was conducted in the task of movement-related activity. This procedure was shown to increase the classification accuracy compared to the conditional common spatial pattern (CSP) algorithm, followed by a linear classifier considered as a baseline approach. In addition, an original approach to finding discriminative temporal segments for each motor imagery was tested. This led to a further increase in accuracy under the conditions of using Hjorth parameters and interchannel correlation coefficients as features calculated for the EEG segments. In particular, classification by the latter feature led to the best accuracy of 71.6%, averaged over all subjects (intrasubject classification), and, surprisingly, it also allowed us to obtain a comparable value of intersubject classification accuracy of 68%. Furthermore, scatter plots demonstrated that two out of three pairs of motor imagery were discriminated by the approach presented.
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