About 60% of the platelets freeze preserved in 6% dimethylsulfoxide retained the discoid shape characteristic of fresh platelets. The remainder were sphered. Platelet sphering occurred during freezing and thawing and was not related to the addition or removal of the cryoprotective agent. Platelets that retained the disc shape had a well-preserved ultrastructure, but sphered platelets showed extensive ultrastructural changes.
Neisseria gonorrhoeae was cultivated in mammalian cell cultures in an effort to determine if this environment will elicit a T4 Ti transition. Of four avirulent (T4) isolates tested, only one, H4, yielded Ti colonies. This change was
the culturcs of lymphoblastoid and leukemic cells of mice, respectively. We also thank Mrs. Margaret M.Rice for assistance in preparing the manuscript.
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