Changes in Platelet Shape and Structure After Freeze Preservation

Crowley, James P.; René, Anthony A.; Valeri, C. R.

doi:10.1182/blood.v44.4.599.599

Cited by 41 publications

(11 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well known that platelet production in combination with PCT with amotosalen and UVA light to inactivate pathogens and WBCs affect platelet quality in different ways. [10][11][12][13][14][15][16] Because freezing and thawing heavily affect the platelets, [23][24][25][26] the initial effects of PCT could lead to additional damage during cryopreservation. Therefore, in vitro characterization of PCT-CPPs in a production line that may increase patient safety is a necessity before clinical studies.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of buffy coat–derived platelet concentrates photochemically treated with amotosalen and UVA light

et al. 2018

View full text Add to dashboard Cite

BACKGROUND Cryopreserved platelets (CPPs) are considered a promising approach for extended platelet storage, bridging inventory shortages of conventionally stored platelets. It is unknown if platelet concentrates exposed to photochemical treatment (PCT) with amotosalen and ultraviolet A (UVA) light, to inactivate pathogens, are suitable for freezing. The objective of this study was to analyze potential effects of PCT on CPPs as compared with untreated CPPs. STUDY DESIGN AND METHODS A total of 12 PCT‐treated and 12 untreated platelet units from buffy coats were cryopreserved at −80°C in 5% dimethyl sulfoxide. CPPs of both types were rapidly thawed at 37°C and resuspended in 200 mL fresh plasma. In vitro properties were analyzed prefreezing, postfreezing and thawing, and on Day 1 after thawing. RESULTS Directly after thawing, no major differences in platelet content, lactase hydrogenase, adenosine triphosphate, mitochondrial membrane potential, CD62P, CD42b, and platelet endothelial cell adhesion molecule were seen between PCT‐CPPs and conventional CPPs. Agonist‐induced PAC‐1 expression and contribution of CPPs to blood coagulation in an experimental rotational thromboelastometry setup were also similar between the groups. On Day 1 after thawing, the CPPs of both types performed less well. The PCT‐CPPs tended to be more affected by the freezing process than the conventional CPPs. CONCLUSIONS PCT‐CPPs appeared slightly more susceptible to lesion effects by freezing than conventional CPPs, in particular in assays on Day 1 after thawing, but these differences were small relative to the dramatic effects of the freezing process itself.

show abstract

Section: Discussionmentioning

confidence: 99%

Cryopreservation of buffy coat–derived platelet concentrates photochemically treated with amotosalen and UVA light

et al. 2018

View full text Add to dashboard Cite

show abstract

“…T he Naval Blood Research Laboratory has conducted numerous PLT studies to evaluate the shape and structure, 1,2 recovery after freezethaw and freeze-thaw-wash procedures, [1][2][3][4][5][6] aggregation, 7 response to hypotonic stress, 8 and sterility. In vitro PLT recovery after the freeze-thaw-wash procedure was found to be about 70 percent, and in vivo recovery was approximately 50 percent that of fresh PLTs.…”

mentioning

confidence: 99%

Effect of WBC reduction and storage temperature on PLTs frozen with 6 percent DMSO for as long as 3 years

et al. 2003

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cryopreservation of PLTs using 6% dimethyl sulfoxide (DMSO) was developed by Valeri's group at the US Navy in the 1970s. 37 The initial approach involved freezing PLTs suspended in DMSO with postthaw removal of the DMSO. Removing most of the DMSO before freezing, allowing omission of any postthaw processing, was found to produce a comparable product 38 with obvious advantages in rapidly delivering the product to the patient with minimal equipment and training required in hospital blood banks.…”

Section: Cryopreserved Pltsmentioning

confidence: 99%

Future of platelet formulations with improved clotting profile: a short review on human safety and efficacy data

Cancelas

2019

Transfusion

View full text Add to dashboard Cite

BACKGROUND Platelet (PLT) transfusion is a widely used therapy in treating or preventing bleeding and hemorrhage in patients with thrombocytopenia or trauma. Compared to the relative ease of PLT transfusion, current practice for PLT storage at room temperature (RT) for up to 5 to 7 days is inefficient, costly, wasteful, and relatively unsafe. STUDY DESIGN AND METHODS This study was a review of major advances in PLT derivative products with improved hemostatic potential and safety feature. RESULTS Recent progress in understanding the PLT activation and host clearance mechanisms has led to reassessments of current and new storage conditions that employ refrigeration and/or cryopreservation to overcome storage lesions and significantly extend shelf life of PLTs with reduced risk of pathogen contamination. DISCUSSION It is anticipated that future PLT preservation involving cold, frozen, and/or pathogen reduction strategies in proper PLT additive solutions will enable longer term and safer PLT storage.

show abstract

Changes in Platelet Shape and Structure After Freeze Preservation

Cited by 41 publications

References 11 publications

Cryopreservation of buffy coat–derived platelet concentrates photochemically treated with amotosalen and UVA light

Cryopreservation of buffy coat–derived platelet concentrates photochemically treated with amotosalen and UVA light

Effect of WBC reduction and storage temperature on PLTs frozen with 6 percent DMSO for as long as 3 years

Future of platelet formulations with improved clotting profile: a short review on human safety and efficacy data

Contact Info

Product

Resources

About