In this study we report sequential genital infections with HSV 1 and HSV 2 in five patients. Patients and methodsThe patients reported here were derived from large groups of patients attending the department of genitourinary medicine, Sheffield, who participated in therapeutic trials of antiviral treatment for genital herpes during 1982-5. During those studies swabs from genital lesions were sent routinely for culture of HSV in tissue, using methods described previously.'0 All isolates of HSV were identified as being HSV 1 or HSV 2 by an enzyme linked immunosorbent assay (ELISA) using either a monoclonal antibody technique, which was described by Nilheden et al " and standardised in our laboratory (Al Samarai, unpublished observation), or a polyclonal antibody technique reported previously.'2To differentiate between primary and non-primary infection we performed microneutralisation tests on

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