Enterococcus faecalis is considered a pathogen responsible for hospital infections and, due to its frequent multi-resistant profile, has caused preoccupations among many medical authorities. The objective of this study was to determine the antimicrobial susceptibility of 74 strains isolated from blood cultures and purulent secretions to vancomycin and ciprofloxacin through the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by using the Microdilution test. The results showed a greater efficacy of vancomycin compared to ciprofloxacin (98.6% of the strains were inhibited by vancomycin at lower concentrations: 0.06 - 1 µg/ml). However, in the MBC analysis 73% of the strains showed a MBC of vancomycin only at high concentrations (equal to or higher than 64 µg/ml). For ciprofloxacin, the strains showed a broad sensitivity with MICs and MBCs distributed along all the MIC classes. Results also revealed a probability that some strains are tolerant to vancomycin, which indicates the need of other tests to confirm this characteristic
Enterococcus faecalis has been considered one of the major agents of nosocomial infection; its resistance to several drugs has been the concern of health authorities. The objective of this study was to determine the antimicrobial susceptibility and presence of mutant strains in Enterococcus faecalis. Seventy-four strains were obtained from hemocultures and purulent secretions of patients hospitalized at the Hospital das Clínicas da Faculdade de Medicina de Botucatu-UNESP-between 1995 and 2001. They were analyzed in vitro for their susceptibility to vancomycin and ciprofloxacin by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and presence of mutant strains by determining the spontaneous mutation rate to ciprofloxacin. Vancomycin showed higher efficacy, inhibiting 98.6% of Enterococcus faecalis strains (MIC 0.06-1.00 µg/ml). However, bactericidal analysis showed 73% of strains with MBC equal or higher than 64 µ/ml. Ciprofloxacin sensitivity profile was observed in MIC and MBC classes. No mutant strains were found in this study. Some strains may be resistant to vancomycin; this requires further testing.
E. coli é a causa mais comum de infecção urinária, sendo responsável por 90%, ou mais, das infecções adquiridas na comunidade, onde sua resistência a diversas drogas tem sido motivo de preocupação das autoridades. O trabalho teve como objetivo comparar a eficácia da ciprofloxacina, ampicilina e ceftriaxona “in vitro” na forma de medicamento de referência e de medicamento genérico. Foram obtidas 79 linhagens provenientes de urina de pacientes com infecção urinária internados no Hospital das Clínicas da Faculdade de Medicina de Botucatu, UNESP, no período de fevereiro a abril de 2004. Foi analisado a suscetibilidade in vitro através da Determinação da Concentração Inibitória Mínima (CIM). Os resultados demonstraram que a ciprofloxacina e a ceftriaxona não apresentaram diferenças significativas entre os medicamentos genéricos e de referência ao contrário do que foi observado com a ampicilina. Esses dados mostram a eficácia dos medicamentos genéricos quando comparados com os de referência.
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