Sperm washing techniques, based on the swim-up principle used before inseminating the human oocyte in in-vitro fertilization and embryo transfer programmes (IVF-ET), usually require prior centrifugation which causes damage to the sperm cell. A technique is described for separating sperm at laboratory temperature based on sperm migration--sedimentation principles, using two concentric tubes and recovering 70-90% forward-moving cells. A group of 17 patients is presented who were managed with this method. The results were 85% fertilization rate, 4% polyspermia and six clinical pregnancies.

Objective: The aim of this study was to compare the results of the semen analysis data while applying the parameters set out in the previous standard and the last proposed, in order to assess the changes in the diagnosis in terms of normal or abnormal, as how this fact can affect treatment decisions. Methods: 1184 semen analysis of patients undergoing examination at our institution were reviewed, between January 2010 and September 2012 and were classified as normal or abnormal according to WHO parameters of 1999 and 2010 to be then compared. Exams of patients over 50 years (39 cases) were excluded for having found in this group a significant increase of asthenozoospermia when compared to younger ages. Results: In the 1145 semen analysis studied, the averadifference was found while analyzing the absolute seminal variables of volume, motility and sperm morphology in the different age groups. Semen parameters in the 1999 were reported as normal with the WHO 2010 compa-ver, the sperm morphology according to the WHO 2010, Conclusion: Based in our data, the sperm analysis as a single tool in the diagnosis of male factor should be revaluated.

A new technique for vaginal intratubal insemination (VITI) and transvaginal gamete intra-Fallopian transfer (TV-GIFT) using endosonography has been developed. A total of 47 infertile couples were treated using a K-JITS 1000-1100 catheter (William Cook, Australia; Jansen Anderson Intratubal Transfer Set) introduced by Jansen and Anderson in Sydney, Australia, and endosonographic control with a Kretz Combison 310 echograph and a 5/7.5 MHz vaginal sectorial transducer. In 40 couples, the VITI technique was used, and in the other seven couples, TV-GIFT was used. Fifteen pregnancies (37.5%) were obtained in 63 VITI cycles (23.8% per treatment cycle) and three with TV-GIFT (42% per treatment cycle). A review is also presented of the migration--sedimentation technique used for management of male factor in an artificial insemination programme.

Presentamos el primer embarazo logrado en nuestro centro de reproducción asistida (CECOLFES) obtenido después de la transferencia de Blastocistos cultivados en medio de Gardner en una paciente de 32 años, con infertilidad secundaria de 8 años de evolución.La paciente fue estimulada con supresión GnRH-a y Gonadotrofina menopáusica humana, realizando la transferencia de 2 blastocistos el día 5 post-aspiración folicular, obteniéndose un embarazo clínico.

No abstract