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Background Chemotherapy has become a standard of treatment in managing breast cancer. To achieve proper treatment for the right patients, the predictive marker is needed. Ki-67 is a biomarker of proliferation for solid tumor. Studies mentioned association of Ki-67 expression with chemotherapy response. The study aims are to evaluate whether Ki-67 expression detected by immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR) may predict clinical response to neoadjuvant chemotherapy in breast cancer. Methods This study utilized a longitudinal study. IHC and qRT-PCR methods were used for detection of Ki-67 expression. Chemotherapy response was calculated using RECIST. Data were analyzed with Chi-square and Wilcoxon's test. Results There were 48 subjects in this study. Analysis of Ki-67 expression with chemotherapy response has a significant correlation with p = 0.025 (<0.05), OR: 1.69, confidence interval (95% CI) 1.022–2.810. Analysis of Ki-67 mRNA expression with chemotherapy response has a significant correlation p = 0.002 (<0.05), OR: 6.85, confidence interval (95% CI) 1.064–44.193. Detection of Ki-67 expression using IHC and qRT-PCR has similar results, p = 0.012 (<0.05). Conclusion These results suggest that Ki-67 expression detected by both IHC and qRT-PCR is considered to be a predictor of clinical response to neoadjuvant chemotherapy in locally advanced breast cancer.

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This study aims to find out (1) the feasibility of the web-based competency test model, and (2) the effectiveness of the web-based competency test model towards increasing the confidence of midwifery students in facing the competency test. Research method using Research and Development (R &D) and Pre-Experiment One Group Pretest-Posttest with Purposive Sampling Technique which is as many as 45 samples. This research was conducted in the Polytechnic of Health Ministry of Makassar and Megarezky University in November 2020. The data is analyzed using statistical analysis by Wilcoxon Test.  Validation test results from media experts averaged 87.9% and validation results from material experts averaged 94% which showed that web competency test model applications are very feasible to use. User validation average feasibility score of 87% of the data showed that the application of web-based competency test model is very feasible to use. The results of the effective use of web-based competency test models found that the Wilcoxon Test obtained a p-value of 0.00 < 0.05. So it can be concluded that the Web-based competency test model is feasible and effective in increasing student confidence facing the competency test.

The physiological parameters included in the Modified Early Obstetric Warning Score (MEOWS) table have been selectively selected so that they cover all the standard observations in the NEWS table with the addition of several parameters that are specifically. The population in this study were all pregnant women who were in RSIA Siti Khadijah I Makassar and RSUP Dr. Wahidin Sudirohusodo Makassar. The results showed that there was no significant difference between the shock index and the incidence of shock (p=0.418). The difference in frequency between the shock index and the incidence of shock in the ICU was 1 patient who was pregnant. Based on shock index with high and low categories did not differ significantly (p=0.418). The data also shows that the frequency of pregnant women who are in shock is higher, namely 44 people (57.9%) compared to those who are not in shock, namely 32 people (42.1%). There was no significant difference between the shock index and the incidence of shock (p=0.112). The difference in frequency between the shock index and the incidence of shock in the ICU was 5 patients with pregnant women. Shock frequency based on the MEOWS index was not significantly different (p=0.112).

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The objective of this research was to investigate serotype diversity pattern of dengue hemorrhagic fever virus by using real-time-polymerase chain reaction (RT-PCR) method. It was an explorative laboratory research in endemic dengue fever area in South Sulawesi Province, Indonesia, that is, Makassar municipality and Maros and Gowa region. Serological examination was carried out using real-time-polymerase chain reaction (RT-PCR) method to determine the serotype of dengue virus. The data showed that, of 30 patients, 20 patients (66.67%) were from Makassar municipality: 10 patients (33.33%) from Gowa region and 10 patients (33.33%) from Maros region. The serotypes found were DENV-2 and DENV-4 and no DENV-1 and DENV-3 serotypes were found. Makassar municipality and Gowa region have higher infection with serotype DENV-2, that is, 40% of cases compared with Maros, which is 20.0%. Statistical test results showed no significant differences between the three endemic areas. Maros region has the highest infection with serotype DENV-4, that is, 40% of cases compared with Makassar municipality (5.0%) and Gowa region (0%). Statistical test results showed significant differences between the three endemic areas. This result revealed that serotypes obtained in endemic areas of dengue fever in South Sulawesi are DENV-2 and DENV-4 and not serotypes DENV-1 and DENV-3. Makassar municipality has DENV-2 and DENV-4 serotype, infection dominated by DENV-2, while Maros region also has DENV-2 and DENV-4, but DENV-4 is the dominant serotype. Gowa municipality only has DENV-2 serotype infection.