Diversity of Dengue Virus Serotype in Endemic Region of South Sulawesi Province

Magzoub

Libyan Journal of Medicine

et al. 2019

Vector-borne diseases are responsible for more than 20% of the infectious diseases worldwide. The prevalence of arboviruses transmit diseases to humans in Sudan has not been investigated. Mosquito-borne viral diseases increase globally incidence, including the Sudan. Frequent unknown fever outbreaks have been reported in eastern region, Sudan. However, diagnosis was based exclusively on clinical signs and symptoms without confirmatory laboratory investigations. However, for accurate detection of these viruses in outbreaks, molecular technique is considered. The objective of this study was to determine the prevalence of six arboviruses in the Kassala state of east Sudan during unknown fever outbreak. A cross sectional hospital-based study was conducted in the Kassala, Teaching Hospital. Blood samples from 119 patients suffering from unknown fever were used for screening of six arboviruses, hepatitis E virus and malarial using molecular techniques and serology. The overall arboviruses seroprevelance was 61.3% (73/119). The highest positivity rate was 73.1% (52/73) chikungunya virus; 29 males and 20 females patients were chikungunya positive. Other arboviruses were circulating in low rate 20.5% (15/73), and 6.8% (5/73) for sindbis and rift valley fever viruses respectively. Hepatitis E virus was negative in all cases and malaria positivity rate 13.4% (16/119). The prevalence of arboviruses among unknown fever patients present to Kassala teaching hospital of eastern region in Sudan is significantly high (61.3%). The chikungunya virus is the predominant causative agent of arboviruses. Molecular techniques such as PCR are important for accurate and rapid diagnosis of this viral outbreak.

Section: Discussionmentioning

confidence: 99%

Prevalence and identification of arthropod-transmitted viruses in Kassala state, Eastern Sudan

Magzoub

Libyan Journal of Medicine

et al. 2019

“…2 Phylogenetic tree based on the level of a similar sequence of 72 DENV-1 strains and 47 reference viruses. The viruses fromVietnam in 2017 are denoted by D1, the strains circulated in Vietnam in the past and in the world are denoted by the country name, GenBank accession number and the year causing the disease DENV types in each of the different epidemics may have the prevalence of multiple dengue serotypes, but only one serotype is dominant [5,[38][39][40].…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing and genetic variations in several dengue virus type 1 strains from unusual dengue epidemic of 2017 in Vietnam

Dang

Pham

Bui

et al. 2020

Virol J

Background: Dengue hemorrhagic fever is an acute viral infection transmitted by mosquitoes. In the 2017, a dengue epidemic occurred in Hanoi in a short time interval and many cases were serious with associated mortality. This was the largest and unusual dengue fever outbreak in the North of Vietnam over the past 20 years. The objective of the present study was to understand the genetic characteristics of the DENV-1 strain in the 2017 epidemic and its relationship with previous viruses in Vietnam and the rest of the world. Methods: Complete genomes of 72 DENV-1 from patients in the 2017 epidemic were sequenced using NGS. The full genome sequences were then analyzed to find out the genetic variants in the groups of 72 strains, followed by their comparison with other strains that caused disease in Vietnam previously and several other regions of the world, revealing a genetic relationship between them. Results: The complete genome sequence of 72 DENV-1 strains comprised 10,697 nucleotides with an open reading frame coding for 3392 amino acids. The genomic analysis revealed different amino acid substitutions in all genes, especially varying at position S75 (Capsid), M125 (PrM), D54 (E), T147, V180 (NS1), G45, Y126, I154 (NS2A), A94 (NS2B), M298 (NS3), K47, V68 (NS4A), I29 (NS4B), and R166, E536, G614, T821 (NS5). The genetic analysis suggested that the viruses were most closely related to the causative virus of the dengue outbreak in Vietnam and Cambodia from 2006 to 2008. These results indicated that DENV-1 from the dengue epidemic 2017 in Northern Vietnam originated from the virus that caused the dengue outbreak during the 2007 to 2008 period in Vietnam. Conclusion: The present study is the first of its kind to describe complete genome sequence as well as genetic variants and phylogenetic analysis of DENV-1 associated with the unusual dengue epidemic of 2017 in northern Vietnam. These results provide detailed evidence to elucidate the origin, circulation, and genetic evolution of DENV in Vietnam.

“…Metode yang digunakan pada tulisan ini adalah literature review dengan metode naratif. 7 9 Faktor risiko lingkungan untuk terjadinya DBD di Kota Semarang tahun 2015 yaitu kelembaban udara sedangkan ketinggian wilayah, pH air, dan suhu udara tidak mempunyai hubungan yang signifikan. Kepadatan jentik juga tidak menunjukkan hubungan dengan kejadian DBD namun tempat penampungan air yang berjentik berhubungan dengan kejadian DBD.…”

Section: Metodeunclassified

Aspek Kekinian tentang Penelitian Demam Berdarah Dengue di Pulau Jawa dan Sekitarnya

Ikawati

2018

blb

Incidence Rate/IR DHF in Indonesia 2015 to 2017 decreased 44.43%, although in 2016 there was an increase of 53.61% from incidence in 2015 (DHF IR per 100,000 population in 2015 until 2017 was 50,75;77,96; 22.55). Five subsystems related to DHF transmission are human, dengue virus, Aedes mosquito, physical and biological environment. Research on these five subsystems and various control efforts has been done in Indonesia. Literature review was used to discuss it in this article. Search area on the site ejournal.litbang.kemkes.go.id, portalgaruda.org, e-resources.perpusnas.go.id, www.researchgate.net, www.hindawi.com and who.int with keywords Aedes aegypti, Dengue Haemorhagic Fever. Several studies showed different results depending on the study site conditions (climatic,altitude,ecological conditions). Human behavior associated with the use of anti-mosquito, dengue virus, Aedes as a vector (potential breeding places, transovary phenomena, insecticide vector resistance), and climate conditions (temperature and humidity) that contribute to the incidence of DHF. Vector control is the most effective measure in DHF control program. The use of Bacillus thuringensis, Romanomermis iyengari, and Wolbachia, the manufactured repellents and larvasides from various plants, the improvement of eradication of mosquito breeding sites related community behavior, and the application of sterile insect techniques have been developed from various studies. The results of such research can be adopted as alternative to control vectors and implemented in integrated manner based on the specific local context.