Using a simple method to introduce genetic modifications into the chromosome of naturally nontransformable Bacillus, a set of marker-free inosine-producing and 5-aminoimidazole-4-carboxamide (AICA) ribonucleoside-producing Bacillus amyloliquefaciens strains has been constructed. These strains differ in expression levels of the genes responsible for nucleoside export. Overexpression of B. amyloliquefaciens pbuE and heterologous expression of Escherichia coli nepI, which encode nucleoside efflux transporters, each notably enhanced inosine production by a B. amyloliquefaciens nucleoside-producing strain. pbuE overexpression was found to increase AICA ribonucleoside accumulation, indicating that the substrate specificity of the PbuE pump extends to this nucleoside. These results demonstrate that identifying genes whose products facilitate transport of a desired nucleoside out of cells and enhancing their expression can improve the performance of strains used for industrial production.
The article is devoted to the definition and systematization of current problems of the logistics system of Ukraine in the context of hostilities and the definition of priorities for its restoration in view of the continuing military conflict in the country. It is substantiated that the high level of logistical threats and the high probability of the further escalation require research in order to find mechanisms to either prevent or at least minimize the risks to the existence of the logistics system of the State. The value of the logistics efficiency index of selected countries for 2018/2019 is analyzed and the disproportion of the components of the index for Ukraine is evidenced given the insufficient quality of trade and transport infrastructure and low efficiency of customs and border clearance. Given the new geopolitical challenges, the need to assess the real state and prospects of resumption of Ukrainian logistics has been proved. The key criteria for the success of the logistics system are systematized, which include: human factor, logistics infrastructure, safety of carriers and cargo, changes in legislation, effectiveness of the partnership. To assess the prospects for the development of the logistics system of the country, which is in a state of active military-political conflict, the index of logistics risk is proposed and it is substantiated that its importance for Ukraine will now reach critical levels. The problems of the logistics system of Ukraine are identified and the ways of their solution are determined, which are systematized by groups: destroyed logistics routes, late deliveries, disproportionate redistribution of goods, risks of uncertainty. It is substantiated that the use of the proposed index of logistics risk of the country will help international businesses and logistics companies to identify priority logistics risks and estimate the likelihood of their further escalation.
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